Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PI 3-kinase p110β CRISPR/Cas9 KO Plasmid (h): sc-400541
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PI 3-kinase p110β CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PI 3-kinase p110β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PI 3-kinase p110β Antibody (C-8): sc-376641
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PI 3-kinase p110β CRISPR/Cas9 KO Plasmid (h) | sc-400541 | 20 µg | $397.00 |
Overview
PIK3CB encodes the p110β catalytic subunit of class I phosphoinositide 3-kinase (PI3K), which phosphorylates PIP2 to generate PIP3 and propagate signals downstream of AKT–mTOR and other effector nodes. PI3K p110β plays a prominent role in signaling initiated by receptor tyrosine kinases and GPCRs, integrating cues that regulate proliferation, survival, metabolism, cytoskeletal dynamics, and vesicular trafficking. In human cells, altered PI3K pathway activity is frequently associated with oncogenic signaling, therapy resistance mechanisms, and aberrant immune and inflammatory responses. PIK3CB function is also relevant to insulin/IGF signaling and cell motility programs, making it a widely studied node in pathway mapping and functional genomics.
PI 3-kinase p110β CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PIK3CB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PIK3CB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PIK3CB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PI 3-kinase p110β protein expression.
This CRISPR knockout system enables efficient generation of PIK3CB-deficient cell models for investigation of PI 3-kinase p110β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PIK3CB exon(s) critical for PI 3-kinase p110β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PIK3CB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PI 3-kinase p110β CRISPR/Cas9 KO Plasmid (h) and PI 3-kinase p110β CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PIK3CB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PI 3-kinase p110β HDR Plasmid (h) and PI 3-kinase p110β HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PIK3CB homology arms to support homology-directed repair at defined PIK3CB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.