PI 3-kinase C2α Antibody (G-5): sc-365290

PI 3-kinase C2α Antibody (G-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects PI 3-kinase C2 alpha in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PI 3-kinase C2α (G-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. PI 3-kinase C2 alpha plays a crucial role in cellular signaling pathways by phosphorylating the 3′ OH position of inositol lipids, which is essential for various cellular processes, including cell growth, proliferation, and survival. Human PI 3-kinase C2 alpha, also known as PIK3C2A, features a C-terminal C2 domain responsible for calcium and phospholipid binding, which is vital for enzymatic activity and interaction with cellular membranes. PI 3-kinase C2 alpha spans approximately 76 kb and consists of 32 exons, with expression detected in a wide range of tissues, indicating a fundamental role in physiological functions. PI 3-kinase C2 alpha′s ability to phosphorylate phosphatidylinositol and phosphatidylinositol-4-phosphate in vitro further underscores its importance in lipid signaling and membrane dynamics, making anti-PI 3-kinase C2α antibody (G-5) an invaluable tool for researchers studying these critical biological processes.

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PI 3-kinase C2α Antibody (G-5) References:

1. Mapping of genes and transcribed sequences in a gene rich 400-kb region on human chromosome 11p15.1-->p14.  |  Caldwell, GM., et al. 2001. Cytogenet Cell Genet. 92: 103-7. PMID: 11306805
2. [The role of phosphatidylinositol-3-kinases P85/P110 and hVPS34 in endocytosis of EGF-receptor complexes].  |  Zheleznova, NN., et al. 2003. Tsitologiia. 45: 574-81. PMID: 14521088
3. Mechanisms regulating phosphoinositide 3-kinase signalling in insulin-sensitive tissues.  |  Shepherd, PR. 2005. Acta Physiol Scand. 183: 3-12. PMID: 15654916
4. Suppression of the alpha-isoform of class II phosphoinositide 3-kinase gene expression leads to apoptotic cell death.  |  Kang, S., et al. 2005. Biochem Biophys Res Commun. 329: 6-10. PMID: 15721265
5. Tryptase activates phosphatidylinositol 3-kinases proteolytically independently from proteinase-activated receptor-2 in cultured dog airway smooth muscle cells.  |  Brown, JK., et al. 2006. Am J Physiol Lung Cell Mol Physiol. 290: L259-69. PMID: 16155087
6. Individual phosphoinositide 3-kinase C2alpha domain activities independently regulate clathrin function.  |  Gaidarov, I., et al. 2005. J Biol Chem. 280: 40766-72. PMID: 16215232
7. The oncogenic properties of mutant p110alpha and p110beta phosphatidylinositol 3-kinases in human mammary epithelial cells.  |  Zhao, JJ., et al. 2005. Proc Natl Acad Sci U S A. 102: 18443-8. PMID: 16339315
8. The evolution of phosphatidylinositol 3-kinases as regulators of growth and metabolism.  |  Engelman, JA., et al. 2006. Nat Rev Genet. 7: 606-19. PMID: 16847462
9. Phosphoinositide 3-kinase C2alpha links clathrin to microtubule-dependent movement.  |  Zhao, Y., et al. 2007. J Biol Chem. 282: 1249-56. PMID: 17110375
10. Phosphoinositide 3-Kinase-C2α Regulates Polycystin-2 Ciliary Entry and Protects against Kidney Cyst Formation.  |  Franco, I., et al. 2016. J Am Soc Nephrol. 27: 1135-44. PMID: 26271513
11. Cpk is a novel class of Drosophila PtdIns 3-kinase containing a C2 domain.  |  Molz, L., et al. 1996. J Biol Chem. 271: 13892-9. PMID: 8662856
12. Cloning of a human phosphoinositide 3-kinase with a C2 domain that displays reduced sensitivity to the inhibitor wortmannin.  |  Domin, J., et al. 1997. Biochem J. 326 (Pt 1): 139-47. PMID: 9337861

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PI 3-kinase C2α Antibody (G-5) has 35 citations in a variety of scientific publications.

Primary antibodies like Anti-PI 3-kinase C2α Antibody (G-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PI 3-kinase C2α Antibody (G-5) Specifications

Alternate Product Name: PI3K C2 alpha

Product Clone Name: G-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 61-360

Epitope Target: PI 3-kinase C2α

Epitope Species: human

Product Reactivity/Detection: PI 3-kinase C2α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 35 citations in various scientific publications

Available Conjugates/Forms for PI 3-kinase C2α Antibody (G-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin