PHIP Antibody (B-1): sc-398614

PHIP Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects PHIP protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PHIP (B-1) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PHIP protein, also known as pleckstrin homology domain interacting protein, ndrp, or WDR11, is a crucial 1,821 amino acid protein characterized by eight N-terminal WD40 repeats and two bromodomains. PHIP protein is predominantly expressed in skeletal muscle, where PHIP localizes to both cytosol and nucleus, as well as in primary β cells, where PHIP is found in the nucleus. PHIP functions as a transcriptional activator and plays a significant role in insulin signaling by interacting with members of the insulin receptor substrate (IRS) family, particularly IRS-1 and IRS-2. These interactions are vital for mediating insulin receptor signaling, which is essential for proliferation and survival of insulin-producing β cells. PHIP′s association with the PH domain of IRS-1 suggests PHIP may serve as a critical link between IRS-1 and insulin receptors, thereby influencing regulation of insulin signaling pathways. Disruptions in the gene encoding PHIP can lead to impaired IRS-mediated signaling, resulting in inhibition of GLUT4 translocation in muscle cells, highlighting PHIP′s importance in metabolic processes. PHIP′s potential interaction with IRS-2 further underscores PHIP′s role in linking insulin receptors to key signaling pathways, making PHIP a significant protein in glucose homeostasis and metabolic health.

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PHIP Antibody (B-1) References:

1. Cloning and characterization of PHIP, a novel insulin receptor substrate-1 pleckstrin homology domain interacting protein.  |  Farhang-Fallah, J., et al. 2000. J Biol Chem. 275: 40492-7. PMID: 11018022
2. The pleckstrin homology (PH) domain-interacting protein couples the insulin receptor substrate 1 PH domain to insulin signaling pathways leading to mitogenesis and GLUT4 translocation.  |  Farhang-Fallah, J., et al. 2002. Mol Cell Biol. 22: 7325-36. PMID: 12242307
3. Insulin receptor substrate 2 plays diverse cell-specific roles in the regulation of glucose transport.  |  Sadagurski, M., et al. 2005. J Biol Chem. 280: 14536-44. PMID: 15705592
4. Role of IRS and PHIP on insulin-induced tyrosine phosphorylation and distribution of IRS proteins.  |  Kaburagi, Y., et al. 2007. Cell Struct Funct. 32: 69-78. PMID: 17634669
5. Identification of a WD40 repeat-containing isoform of PHIP as a novel regulator of beta-cell growth and survival.  |  Podcheko, A., et al. 2007. Mol Cell Biol. 27: 6484-96. PMID: 17636024
6. Dok1 mediates high-fat diet-induced adipocyte hypertrophy and obesity through modulation of PPAR-gamma phosphorylation.  |  Hosooka, T., et al. 2008. Nat Med. 14: 188-93. PMID: 18204460

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PHIP Antibody (B-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PHIP Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PHIP Antibody (B-1) Specifications

Alternate Product Name: PHIP

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1470-1771

Epitope Target: PHIP

Epitope Species: human

Product Reactivity/Detection: PHIP

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PHIP Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin