PHI-1 Antibody (D-8): sc-514759

PHI-1 Antibody (D-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects PHI-1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PHI-1 (D-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PHI-1, also known as PPP1R14B (protein phosphatase 1, regulatory (inhibitor) subunit 14B), PLCB3N (phospholipase C-beta-3 neighboring gene protein), PNG, or SOM172, is a 147 amino acid coiled-coil protein that plays a crucial role in cellular signaling by inhibiting protein phosphatase 1 (PP1). This inhibition is vital as PP1 is involved in various cellular processes, including cell division, metabolism, and muscle contraction. PHI-1 is primarily located in the cytoplasm and is expressed at low levels across different tissues, where PHI-1 functions to block the dephosphorylation of myosin light chains, thereby influencing cell migration and adhesion. Notably, PHI-1 interacts with MYPT1, a significant regulator that may act as a tumor suppressor by modulating Rho-dependent amoeboid cell behavior during metastasis. PHI-1 undergoes post-translational modifications, particularly phosphorylation on threonine 57 and an unidentified serine by protein kinase C, which enhances inhibitory activity by 50-fold, underscoring the importance of these modifications in regulating function. The gene encoding PHI-1 is located on human chromosome 11q13.1, highlighting relevance in both normal physiology and potential pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PHI-1 Antibody (D-8) References:

1. Regulator-driven functional diversification of protein phosphatase-1 in eukaryotic evolution.  |  Ceulemans, H., et al. 2002. Bioessays. 24: 371-81. PMID: 11948623
2. A preoperative diagnostic test that distinguishes benign from malignant thyroid carcinoma based on gene expression.  |  Cerutti, JM., et al. 2004. J Clin Invest. 113: 1234-42. PMID: 15085203
3. Families of protein phosphatase 1 modulators activated by protein kinases a and C: focus on brain.  |  Liu, QR., et al. 2005. Prog Nucleic Acid Res Mol Biol. 79: 371-404. PMID: 16096033
4. Rho-regulated myosin phosphatase establishes the level of protrusive activity required for cell movements during zebrafish gastrulation.  |  Weiser, DC., et al. 2009. Development. 136: 2375-84. PMID: 19515695
5. Effect of KEPI (Ppp1r14c) deletion on morphine analgesia and tolerance in mice of different genetic backgrounds: when a knockout is near a relevant quantitative trait locus.  |  Drgonova, J., et al. 2010. Neuroscience. 165: 882-95. PMID: 19819304
6. Sequence and expression of the mouse homologue to human phospholipase C beta3 neighboring gene.  |  Lagercrantz, J., et al. 1996. Biochem Biophys Res Commun. 223: 335-40. PMID: 8670283
7. Isolation and characterization of a novel gene close to the human phosphoinositide-specific phospholipase C beta 3 gene on chromosomal region 11q13.  |  Lagercrantz, J., et al. 1996. Genomics. 31: 380-4. PMID: 8838322

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PHI-1 Antibody (D-8) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PHI-1 Antibody (D-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PHI-1 Antibody (D-8) Specifications

Alternate Product Name: PHI-1

Product Clone Name: D-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 2-41

Epitope Target: PHI-1

Epitope Species: human

Product Reactivity/Detection: PHI-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PHI-1 Antibody (D-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin