Anti-PHI-1 Antibody (C-11) recommended for detection of PHI-1 of mouse, rat and human origin by WB, IP, IF and ELISA

Anti-PHI-1 Antibody (C-11): sc-514811

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Datasheets

    • PHI-1 Antibody (C-11) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 2-41 mapping near the C-terminus of PHI-1 of human origin
    • recommended for detection of PHI-1 of mouse, rat and human origin by WB, IP, IF and ELISA
    • m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for PHI-1 Antibody (C-11) for WB applications. These reagents are now offered in bundles with PHI-1 Antibody (C-11) (see ordering information below).
loading

How much sample do you recommend loading on the gel for Western blot detection using PHI-1 (C-11): sc-514811?

Asked by: TinTin
We recommend loading 40–60 µg whole cell lysate, 10–20 µg nuclear extract, 5-10 µg transfected lysate, or 10–20 ng purified protein per lane. A link to our complete Western blotting protocol is provided here for your reference: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-01
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