PHACTR3 Antibody (H-3): sc-390843
- PHACTR3 Antibody (H-3) is a mouse monoclonal IgG1 κ PHACTR3 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 257-244 within an internal region of PHACTR3 of human origin
- PHACTR3 Antibody (H-3) is recommended for detection of PHACTR3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PHACTR3 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PHACTR3 Antibody (H-3) for WB applications. These reagents are now offered in bundles with PHACTR3 Antibody (H-3) (see ordering information below).
QUICK LINKS
PHACTR3 Antibody (H-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PHACTR3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PHACTR3 antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PHACTR3, also known as phosphatase and actin regulator 3 or scapinin, is a crucial nuclear matrix protein composed of 559 amino acids that plays a significant role in cellular signaling by inhibiting protein phosphatase 1 (PP1) activity through interactions with actin and PP1α catalytic subunit. This inhibition is vital for regulating various cellular functions, including cell division, homeostasis, and apoptosis, as PHACTR3 modulates phosphorylation state of target proteins. PHACTR3 is predominantly expressed in brain, with lower expression levels found in placenta, thymus, and spleen. Notably, mutations in PHACTR3 gene have been implicated in non-small cell lung carcinoma, correlating with poorer prognosis, and PHACTR3 is upregulated in several tumor types, particularly isoform 3, which is a major form expressed in GOTO, HL-60, and U937 cell lines. Additionally, PHACTR3 exists in three isoforms due to alternative splicing, highlighting complex regulatory roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PHACTR3 Antibody (H-3) References:
- Scapinin, a putative protein phosphatase-1 regulatory subunit associated with the nuclear nonchromatin structure. | Sagara, J., et al. 2003. J Biol Chem. 278: 45611-9. PMID: 12925532
- Phactrs 1-4: A family of protein phosphatase 1 and actin regulatory proteins. | Allen, PB., et al. 2004. Proc Natl Acad Sci U S A. 101: 7187-92. PMID: 15107502
- Genomic organization and expression pattern of scapinin (PHACTR3) in mouse and human. | Worch, S., et al. 2006. Cytogenet Genome Res. 115: 23-9. PMID: 16974080
- Quantitative phosphoproteome profiling of Wnt3a-mediated signaling network: indicating the involvement of ribonucleoside-diphosphate reductase M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal transduction. | Tang, LY., et al. 2007. Mol Cell Proteomics. 6: 1952-67. PMID: 17693683
- Paramutation-like effects at the mouse scapinin (Phactr3) locus. | Worch, S., et al. 2008. J Mol Biol. 377: 605-8. PMID: 18295233
- Investigation of QTL regions on Chromosome 17 for genes associated with meat color in the pig. | Fan, B., et al. 2008. J Anim Breed Genet. 125: 240-7. PMID: 18717966
- Scapinin, the protein phosphatase 1 binding protein, enhances cell spreading and motility by interacting with the actin cytoskeleton. | Sagara, J., et al. 2009. PLoS One. 4: e4247. PMID: 19158953
- Identification of genes associated with non-small-cell lung cancer promotion and progression. | Bankovic, J., et al. 2010. Lung Cancer. 67: 151-9. PMID: 19473719
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PHACTR3 Antibody (H-3) | sc-390843 | 200 µg/ml | $322.00 | |||
PHACTR3 Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-530281 | 200 µg Ab; 10 µg BP | $361.00 | |||
PHACTR3 Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-523703 | 200 µg Ab, 40 µg BP | $361.00 | |||
PHACTR3 Antibody (H-3) AC | sc-390843 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PHACTR3 Antibody (H-3) HRP | sc-390843 HRP | 200 µg/ml | $322.00 | |||
PHACTR3 Antibody (H-3) FITC | sc-390843 FITC | 200 µg/ml | $336.00 | |||
PHACTR3 Antibody (H-3) PE | sc-390843 PE | 200 µg/ml | $349.00 | |||
PHACTR3 Antibody (H-3) Alexa Fluor® 488 | sc-390843 AF488 | 200 µg/ml | $364.00 | |||
PHACTR3 Antibody (H-3) Alexa Fluor® 546 | sc-390843 AF546 | 200 µg/ml | $364.00 | |||
PHACTR3 Antibody (H-3) Alexa Fluor® 594 | sc-390843 AF594 | 200 µg/ml | $364.00 | |||
PHACTR3 Antibody (H-3) Alexa Fluor® 647 | sc-390843 AF647 | 200 µg/ml | $364.00 | |||
PHACTR3 Antibody (H-3) Alexa Fluor® 680 | sc-390843 AF680 | 200 µg/ml | $364.00 | |||
PHACTR3 Antibody (H-3) Alexa Fluor® 790 | sc-390843 AF790 | 200 µg/ml | $364.00 | |||
PHACTR3 (H-3) Neutralizing Peptide | sc-390843 P | 100 µg/0.5 ml | $69.00 |