PGRP-L Antibody (B-4): sc-166646
- PGRP-L Antibody (B-4) is a mouse monoclonal IgG1 κ PGRP-L antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 22-321 mapping near the N-terminus of PGRP-L of human origin
- PGRP-L Antibody (B-4) is recommended for detection of PGRP-L of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-PGRP-L Antibody (B-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PGRP-L Antibody (B-4) for WB and IHC(P) applications. These reagents are now offered in bundles with PGRP-L Antibody (B-4) (see ordering information below).
PGRP-L Antibody (B-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects PGRP-L of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PGRP-L antibody (B-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Peptidoglycan recognition protein-L (PGRP-L) is a crucial pattern recognition molecule composed of 576 amino acids, making PGRP-L the longest and most widely expressed member of the PGRP family. PGRP-L plays a significant role in innate immune response by recognizing and binding to peptidoglycan, a component of bacterial cell walls, which is essential for immune system detection and response to bacterial infections. PGRP-L exhibits N-acetylmuramyl-L-alanine-amidase activity, allowing peptidoglycan digestion, and can form homodimers as well as dimerize with other PGRP proteins. While primarily a secreted serum protein, PGRP-L is also expressed as a transmembrane protein in liver parenchymal cells, further enhancing immune surveillance. PGRP-L deficient mice show slightly reduced levels of interleukin-6 and tumor necrosis factor-alpha, indicating a potential but minor role in innate immune functions. PGRP-L may be instrumental in activating Nod1, an intracellular pattern recognition receptor, highlighting PGRP-L′s importance in broader immune signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PGRP-L Antibody (B-4) References:
- Peptidoglycan recognition proteins: a novel family of four human innate immunity pattern recognition molecules. | Liu, C., et al. 2001. J Biol Chem. 276: 34686-94. PMID: 11461926
- NMR structure of Citrobacter freundii AmpD, comparison with bacteriophage T7 lysozyme and homology with PGRP domains. | Liepinsh, E., et al. 2003. J Mol Biol. 327: 833-42. PMID: 12654266
- Peptidoglycan molecular requirements allowing detection by Nod1 and Nod2. | Girardin, SE., et al. 2003. J Biol Chem. 278: 41702-8. PMID: 12871942
- Human peptidoglycan recognition protein-L is an N-acetylmuramoyl-L-alanine amidase. | Wang, ZM., et al. 2003. J Biol Chem. 278: 49044-52. PMID: 14506276
- Peptidoglycan recognition protein expression in mouse Peyer's Patch follicle associated epithelium suggests functional specialization. | Lo, D., et al. 2003. Cell Immunol. 224: 8-16. PMID: 14572796
- Human exposure to 4.0-Tesla magnetic fields in a whole-body scanner. | Schenck, JF., et al. 1992. Med Phys. 19: 1089-98. PMID: 1518472
- Innate immune responses in peptidoglycan recognition protein L-deficient mice. | Xu, M., et al. 2004. Mol Cell Biol. 24: 7949-57. PMID: 15340057
- Chemically synthesized pathogen-associated molecular patterns increase the expression of peptidoglycan recognition proteins via toll-like receptors, NOD1 and NOD2 in human oral epithelial cells. | Uehara, A., et al. 2005. Cell Microbiol. 7: 675-86. PMID: 15839897
- Downregulation of the Drosophila immune response by peptidoglycan-recognition proteins SC1 and SC2. | Bischoff, V., et al. 2006. PLoS Pathog. 2: e14. PMID: 16518472
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PGRP-L Antibody (B-4) | sc-166646 | 200 µg/ml | $322.00 | |||
PGRP-L Antibody (B-4): m-IgG Fc BP-HRP Bundle | sc-529017 | 200 µg Ab; 10 µg BP | $361.00 | |||
PGRP-L Antibody (B-4): m-IgGκ BP-HRP Bundle | sc-521690 | 200 µg Ab, 40 µg BP | $361.00 | |||
PGRP-L Antibody (B-4): m-IgG1 BP-HRP Bundle | sc-543270 | 200 µg Ab; 20 µg BP | $361.00 | |||
PGRP-L Antibody (B-4) AC | sc-166646 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PGRP-L Antibody (B-4) HRP | sc-166646 HRP | 200 µg/ml | $322.00 | |||
PGRP-L Antibody (B-4) FITC | sc-166646 FITC | 200 µg/ml | $336.00 | |||
PGRP-L Antibody (B-4) PE | sc-166646 PE | 200 µg/ml | $349.00 | |||
PGRP-L Antibody (B-4) Alexa Fluor® 488 | sc-166646 AF488 | 200 µg/ml | $364.00 | |||
PGRP-L Antibody (B-4) Alexa Fluor® 546 | sc-166646 AF546 | 200 µg/ml | $364.00 | |||
PGRP-L Antibody (B-4) Alexa Fluor® 594 | sc-166646 AF594 | 200 µg/ml | $364.00 | |||
PGRP-L Antibody (B-4) Alexa Fluor® 647 | sc-166646 AF647 | 200 µg/ml | $364.00 | |||
PGRP-L Antibody (B-4) Alexa Fluor® 680 | sc-166646 AF680 | 200 µg/ml | $364.00 | |||
PGRP-L Antibody (B-4) Alexa Fluor® 790 | sc-166646 AF790 | 200 µg/ml | $364.00 |