PGLS Antibody (G-7): sc-398833

PGLS Antibody (G-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects PGLS protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PGLS Antibody (G-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PGLS, or 6-phosphogluconolactonase, is a 258 amino acid enzyme that plays a crucial role in the pentose phosphate pathway by catalyzing the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate, a key intermediate in cellular metabolism. This pathway is essential for generating NADPH and ribose-5-phosphate, which are vital for anabolic reactions and nucleotide synthesis, respectively. The enzyme is localized in the cytoplasm, where PGLS is particularly active, and PGLS′s highly conserved nature allows significant sequence similarity with various homologs across species, including yeast and bacteria. Notably, PGLS deficiency can lead to hemolytic anemia when combined with glucose-6-phosphate dehydrogenase deficiency, highlighting PGLS′s importance in maintaining red blood cell integrity. The gene encoding PGLS is located on human chromosome 19p13.11, underscoring PGLS′s relevance in human health and disease.

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PGLS Antibody (G-7) References:

1. Identification of the cDNA encoding human 6-phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway(1).  |  Collard, F., et al. 1999. FEBS Lett. 459: 223-6. PMID: 10518023
2. NMR spectroscopic analysis of the first two steps of the pentose-phosphate pathway elucidates the role of 6-phosphogluconolactonase.  |  Miclet, E., et al. 2001. J Biol Chem. 276: 34840-6. PMID: 11457850
3. Identification by redox proteomics of glutathionylated proteins in oxidatively stressed human T lymphocytes.  |  Fratelli, M., et al. 2002. Proc Natl Acad Sci U S A. 99: 3505-10. PMID: 11904414
4. Identification of extracellular and intracellular signaling components of the mammary adipose tissue and its interstitial fluid in high risk breast cancer patients: toward dissecting the molecular circuitry of epithelial-adipocyte stromal cell interactions.  |  Celis, JE., et al. 2005. Mol Cell Proteomics. 4: 492-522. PMID: 15695426
5. 6-Phosphogluconolactonase deficiency, a hereditary erythrocyte enzyme deficiency: possible interaction with glucose-6-phosphate dehydrogenase deficiency.  |  Beutler, E., et al. 1985. Proc Natl Acad Sci U S A. 82: 3876-8. PMID: 3858849
6. Glucose metabolism in 6 phosphogluconolactonase mutants of Escherichia coli.  |  Kupor, SR. and Fraenkel, DG. 1972. J Biol Chem. 247: 1904-10. PMID: 4552019
7. 6-phosphogluconolactonase mutants of Escherichia coli and a maltose blue gene.  |  Kupor, SR. and Fraenkel, DG. 1969. J Bacteriol. 100: 1296-301. PMID: 4902810

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PGLS Antibody (G-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PGLS Antibody (G-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PGLS Antibody (G-7) Specifications

Alternate Product Name: PGLS

Product Clone Name: G-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 96-123

Epitope Target: PGLS

Epitope Species: human

Product Reactivity/Detection: PGLS

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PGLS Antibody (G-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin