PGAM5 Antibody (A-3): sc-515880
- PGAM5 Antibody (A-3) is a mouse monoclonal IgG1 κ PGAM5 antibody, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 115-239 D196 mapping near the C-terminus of PGAM5 of human origin
- PGAM5 Antibody (A-3) is recommended for detection of PGAM5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PGAM5 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PGAM5 Antibody (A-3) for WB applications. These reagents are now offered in bundles with PGAM5 Antibody (A-3) (see ordering information below).
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PGAM5 Antibody (A-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PGAM5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PGAM5 (A-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PGAM5, also known as phosphoglycerate mutase family member 5, plays a crucial role in cellular metabolism, particularly in the regulation of glucose and 2,3-bisphosphoglycerate metabolism, by catalyzing the transfer of phosphate groups between phosphoglycerate molecules. This enzymatic activity is vital for maintaining energy homeostasis and supporting various metabolic pathways, which are essential for cell survival and function. PGAM5 is a 289 amino acid protein that exists in two isoforms due to alternative splicing, with one isoform localized to the cytoplasm and the other found in both the cytoplasm and nucleus. PGAM5 forms dimers and has been shown to interact with key regulatory proteins such as Bcl-xS/L and Keap1, highlighting its importance in cellular signaling and stress response mechanisms.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PGAM5 Antibody (A-3) References:
- Mouse phosphoglycerate mutase M and B isozymes: cDNA cloning, enzyme activity assay and mapping. | Zhang, J., et al. 2001. Gene. 264: 273-9. PMID: 11250083
- In vitro selection and characterization of Bcl-X(L)-binding proteins from a mix of tissue-specific mRNA display libraries. | Hammond, PW., et al. 2001. J Biol Chem. 276: 20898-906. PMID: 11283018
- Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization. | Jin, J., et al. 2004. Curr Biol. 14: 1436-50. PMID: 15324660
- Glycolysis in Entamoeba histolytica. Biochemical characterization of recombinant glycolytic enzymes and flux control analysis. | Saavedra, E., et al. 2005. FEBS J. 272: 1767-83. PMID: 15794763
- Characterization of the first described mutation of human red blood cell phosphoglycerate mutase. | de Atauri, P., et al. 2005. Biochim Biophys Acta. 1740: 403-10. PMID: 15949708
- PGAM5, a Bcl-XL-interacting protein, is a novel substrate for the redox-regulated Keap1-dependent ubiquitin ligase complex. | Lo, SC. and Hannink, M. 2006. J Biol Chem. 281: 37893-903. PMID: 17046835
- PGAM5 regulates PINK1/Parkin-mediated mitophagy via DRP1 in CCCP-induced mitochondrial dysfunction. | Park, YS., et al. 2018. Toxicol Lett. 284: 120-128. PMID: 29241732
- PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis. | Yan, C., et al. 2020. Autophagy. 16: 419-434. PMID: 31177901
- Mitochondrial phosphatase PGAM5 modulates cellular senescence by regulating mitochondrial dynamics. | Yu, B., et al. 2020. Nat Commun. 11: 2549. PMID: 32439975
- PGAM5 is an MFN2 phosphatase that plays an essential role in the regulation of mitochondrial dynamics. | Nag, S., et al. 2023. Cell Rep. 42: 112895. PMID: 37498743
- The E3 ubiquitin ligase MARCH2 protects against myocardial ischemia-reperfusion injury through inhibiting pyroptosis via negative regulation of PGAM5/MAVS/NLRP3 axis. | Liu, S., et al. 2024. Cell Discov. 10: 24. PMID: 38409220
- Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis. | Liu, Y., et al. 2024. Bone Res. 12: 15. PMID: 38433252
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PGAM5 Antibody (A-3) | sc-515880 | 200 µg/ml | $316.00 | |||
PGAM5 Antibody (A-3): m-IgG Fc BP-HRP Bundle | sc-531547 | 200 µg Ab; 10 µg BP | $354.00 | |||
PGAM5 Antibody (A-3): m-IgGκ BP-HRP Bundle | sc-525263 | 200 µg Ab, 40 µg BP | $354.00 | |||
PGAM5 Antibody (A-3) AC | sc-515880 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PGAM5 Antibody (A-3) HRP | sc-515880 HRP | 200 µg/ml | $316.00 | |||
PGAM5 Antibody (A-3) FITC | sc-515880 FITC | 200 µg/ml | $330.00 | |||
PGAM5 Antibody (A-3) PE | sc-515880 PE | 200 µg/ml | $343.00 | |||
PGAM5 Antibody (A-3) Alexa Fluor® 488 | sc-515880 AF488 | 200 µg/ml | $357.00 | |||
PGAM5 Antibody (A-3) Alexa Fluor® 546 | sc-515880 AF546 | 200 µg/ml | $357.00 | |||
PGAM5 Antibody (A-3) Alexa Fluor® 594 | sc-515880 AF594 | 200 µg/ml | $357.00 | |||
PGAM5 Antibody (A-3) Alexa Fluor® 647 | sc-515880 AF647 | 200 µg/ml | $357.00 | |||
PGAM5 Antibody (A-3) Alexa Fluor® 680 | sc-515880 AF680 | 200 µg/ml | $357.00 | |||
PGAM5 Antibody (A-3) Alexa Fluor® 790 | sc-515880 AF790 | 200 µg/ml | $357.00 |