PFAAP5 Antibody (F-2): sc-514596
- PFAAP5 Antibody (F-2) is a mouse monoclonal IgG1 κ PFAAP5 antibody provided at 200 µg/ml
- raised against amino acids 489-550 mapping near the C-terminus of PFAAP5 of human origin
- PFAAP5 Antibody (F-2) is recommended for detection of PFAAP5 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PFAAP5 Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PFAAP5 Antibody (F-2) for WB applications. These reagents are now offered in bundles with PFAAP5 Antibody (F-2) (see ordering information below).
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PFAAP5 Antibody (F-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects PFAAP5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PFAAP5 (F-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PFAAP5 protein, also known as phosphonoformate immuno-associated protein 5 or NEDD4-binding protein 2-like 2, is a 583 amino acid nuclear protein that plays a crucial role in transcriptional regulation, particularly in response to DNA damage. PFAAP5 is phosphorylated on serine 199, likely by ATM or ATR kinases, which is essential for function in cellular stress responses. Primarily expressed in bone marrow, PFAAP5 is significantly down-regulated following exposure to arsenic compounds, a change that precedes the onset of neutropenia, highlighting importance in hematopoiesis and immune response. PFAAP5 interacts with Gfi-1 and Neutrophil Elastase, both implicated in neutropenia disorders. Mutations in the gene encoding Neutrophil Elastase, ELA2, lead to cyclic hematopoiesis, characterized by reduced neutrophil counts and increased susceptibility to opportunistic infections. Gfi1 acts as a transcriptional repressor targeting the ELA2 gene, and PFAAP5 can enhance the repression of Gfi1 target genes, facilitating the transcription of ELA2 and promoting neutrophil differentiation. Two isoforms of PFAAP5 arise from alternative splicing, further diversifying functional roles in cellular processes.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PFAAP5 Antibody (F-2) References:
- Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells. | Doan, LL., et al. 2004. Nucleic Acids Res. 32: 2508-19. PMID: 15131254
- Gene expression profiles in peripheral lymphocytes by arsenic exposure and skin lesion status in a Bangladeshi population. | Argos, M., et al. 2006. Cancer Epidemiol Biomarkers Prev. 15: 1367-75. PMID: 16835338
- ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. | Matsuoka, S., et al. 2007. Science. 316: 1160-6. PMID: 17525332
- Clinical implications of ELA2-, HAX1-, and G-CSF-receptor (CSF3R) mutations in severe congenital neutropenia. | Zeidler, C., et al. 2009. Br J Haematol. 144: 459-67. PMID: 19120359
- Ela2 mutations and clinical manifestations in familial congenital neutropenia. | Shiohara, M., et al. 2009. J Pediatr Hematol Oncol. 31: 319-24. PMID: 19415009
- Contributions to neutropenia from PFAAP5 (N4BP2L2), a novel protein mediating transcriptional repressor cooperation between Gfi1 and neutrophil elastase. | Salipante, SJ., et al. 2009. Mol Cell Biol. 29: 4394-405. PMID: 19506020
- Generation of an integrated transcription map of the BRCA2 region on chromosome 13q12-q13. | Couch, FJ., et al. 1996. Genomics. 36: 86-99. PMID: 8812419
- The Gfi-1 protooncoprotein represses Bax expression and inhibits T-cell death. | Grimes, HL., et al. 1996. Proc Natl Acad Sci U S A. 93: 14569-73. PMID: 8962093
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PFAAP5 Antibody (F-2) | sc-514596 | 200 µg/ml | $322.00 | |||
PFAAP5 Antibody (F-2): m-IgG Fc BP-HRP Bundle | sc-531173 | 200 µg Ab; 10 µg BP | $361.00 | |||
PFAAP5 Antibody (F-2): m-IgGκ BP-HRP Bundle | sc-524786 | 200 µg Ab, 40 µg BP | $361.00 | |||
PFAAP5 Antibody (F-2): m-IgG1 BP-HRP Bundle | sc-544422 | 200 µg Ab; 20 µg BP | $361.00 | |||
PFAAP5 Antibody (F-2) AC | sc-514596 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PFAAP5 Antibody (F-2) HRP | sc-514596 HRP | 200 µg/ml | $322.00 | |||
PFAAP5 Antibody (F-2) FITC | sc-514596 FITC | 200 µg/ml | $336.00 | |||
PFAAP5 Antibody (F-2) PE | sc-514596 PE | 200 µg/ml | $349.00 | |||
PFAAP5 Antibody (F-2) Alexa Fluor® 488 | sc-514596 AF488 | 200 µg/ml | $364.00 | |||
PFAAP5 Antibody (F-2) Alexa Fluor® 546 | sc-514596 AF546 | 200 µg/ml | $364.00 | |||
PFAAP5 Antibody (F-2) Alexa Fluor® 594 | sc-514596 AF594 | 200 µg/ml | $364.00 | |||
PFAAP5 Antibody (F-2) Alexa Fluor® 647 | sc-514596 AF647 | 200 µg/ml | $364.00 | |||
PFAAP5 Antibody (F-2) Alexa Fluor® 680 | sc-514596 AF680 | 200 µg/ml | $364.00 | |||
PFAAP5 Antibody (F-2) Alexa Fluor® 790 | sc-514596 AF790 | 200 µg/ml | $364.00 |