PF-4 Antibody (PF63.1): sc-73638

PF-4 Antibody (PF63.1) is a mouse monoclonal IgG1 antibody that detects platelet factor-4 (PF-4) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). PF-4 is a 70 amino acid protein released from the alpha-granules of activated platelets, playing a crucial role in hemostasis by binding with high affinity to heparin and related sulfated glycosaminoglycans. This interaction is vital as PF-4 neutralizes heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting the activity of antithrombin III and promoting coagulation. Additionally, PF-4 acts as a potent chemoattractant for neutrophils and fibroblasts, suggesting involvement in inflammation and wound healing processes. Notably, studies have shown that PF-4 expression is significantly elevated in the spleens of adult NOA mice, an animal model for allergic dermatitis, compared to younger mice, indicating a potential role in the pathogenesis of atopic dermatitis. The gene encoding PF-4 is located on human chromosome 4q13.3 and is classified as a small inducible gene, reflecting its small size and the stimulation undergone during platelet activation. Anti-PF-4 antibody (PF63.1) is an essential tool for researchers investigating the multifaceted roles of PF-4 in coagulation and inflammatory responses.

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PF-4 Antibody (PF63.1) References:

1. Platelet factor 4 (PF-4)-induced neutrophil adhesion is controlled by src-kinases, whereas PF-4-mediated exocytosis requires the additional activation of p38 MAP kinase and phosphatidylinositol 3-kinase.  |  Kasper, B., et al. 2004. Blood. 103: 1602-10. PMID: 14592823
2. PF-4/CXCL4 and CXCL4L1 exhibit distinct subcellular localization and a differentially regulated mechanism of secretion.  |  Lasagni, L., et al. 2007. Blood. 109: 4127-34. PMID: 17218382
3. Effect of allergen-specific immunotherapy on plasma level of platelet factor 4 (PF-4) and beta-thromboglobulin (beta-TG), platelet activation markers in patients with house dust mite allergy.  |  Kasperska-Zajac, A., et al. 2007. Vaccine. 25: 3595-8. PMID: 17291641
4. Recovery of platelet factor 4 (PF-4) and beta-thromboglobulin (beta-TG) plasma concentrations during remission in patients suffering from atopic dermatitis.  |  Kasperska-Zajac, A. 2010. Platelets. 21: 522-4. PMID: 20670105
5. The role of the CXC chemokines platelet factor-4 (CXCL4/PF-4) and its variant (CXCL4L1/PF-4var) in inflammation, angiogenesis and cancer.  |  Vandercappellen, J., et al. 2011. Cytokine Growth Factor Rev. 22: 1-18. PMID: 21111666
6. Platelet-derived chemokines, PF-4 and RANTES, are significantly increased in hemodynamically significant degenerative aortic stenosis.  |  Motovska, Z., et al. 2011. Int J Cardiol. 152: 273-5. PMID: 21893355
7. Role of platelet chemokines, PF-4 and CTAP-III, in cancer biology.  |  Pilatova, K., et al. 2013. J Hematol Oncol. 6: 42. PMID: 23800319
8. Vascular endothelial growth factor (VEGF) and platelet (PF-4) factor 4 inputs modulate human microvascular endothelial signaling in a three-dimensional matrix migration context.  |  Hang, TC., et al. 2013. Mol Cell Proteomics. 12: 3704-18. PMID: 24023389
9. IL-6, PF-4, sCD40 L, and homocysteine are associated with the radiological progression of cerebral small-vessel disease: a 2-year follow-up study.  |  Staszewski, J., et al. 2018. Clin Interv Aging. 13: 1135-1141. PMID: 29950823
10. Natural history of anti-PF 4 antibodies in patients with vaccine-induced immune thrombocytopenia and thrombosis.  |  Lotti, E., et al. 2024. Blood Transfus. 22: 246-252. PMID: 38315531

The preferred PF-4 antibody is PF-4 Antibody (D-7): sc-398979.