pescadillo Antibody (H-10): sc-166300
- pescadillo Antibody (H-10) is a mouse monoclonal IgG1 κ pescadillo antibody, cited in 5 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 399-427 near the C-terminus of pescadillo of human origin
- pescadillo Antibody (H-10) is recommended for detection of pescadillo isoforms 1 and 2 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-pescadillo Antibody (H-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- TransCruz reagent for ChIP application (sc-166300 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for pescadillo Antibody (H-10) for WB applications. These reagents are now offered in bundles with pescadillo Antibody (H-10) (see ordering information below).
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Pescadillo Antibody (H-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects pescadillo protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-pescadillo antibody (H-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pescadillo, also known as PES1, consists of 588 amino acids and is the human homolog of zebrafish pescadillo, exhibiting 74% sequence identity with its zebrafish counterpart. PES1 plays a crucial role in cell cycle regulation and is essential for proper embryonic development, particularly during early stages of zebrafish development where PES1 is highly expressed. Notably, PES1 is predominantly found in liver, kidney, and testis in adult mouse tissues, while PES1 expression is minimal in skeletal muscle. PES1 upregulation has been observed in human breast carcinoma and glioblastoma cells, indicating potential involvement in tumorigenesis. Additionally, proliferation in HeLa cells is contingent upon PES1 expression, highlighting PES1 significance in cellular growth and division.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
pescadillo Antibody (H-10) References:
- The DNA sequence of human chromosome 22. | Dunham, I., et al. 1999. Nature. 402: 489-95. PMID: 10591208
- Pescadillo, a novel cell cycle regulatory protein abnormally expressed in malignant cells. | Kinoshita, Y., et al. 2001. J Biol Chem. 276: 6656-65. PMID: 11071894
- The murine Pes1 gene encodes a nuclear protein containing a BRCT domain. | Haque, J., et al. 2000. Genomics. 70: 201-10. PMID: 11112348
- Pescadillo is essential for nucleolar assembly, ribosome biogenesis, and mammalian cell proliferation. | Lerch-Gaggl, A., et al. 2002. J Biol Chem. 277: 45347-55. PMID: 12237316
- Role of pescadillo and upstream binding factor in the proliferation and differentiation of murine myeloid cells. | Prisco, M., et al. 2004. Mol Cell Biol. 24: 5421-33. PMID: 15169904
- Role of pescadillo in the transformation and immortalization of mammalian cells. | Maiorana, A., et al. 2004. Oncogene. 23: 7116-24. PMID: 15273728
- Inactivation of the RRB1-Pescadillo pathway involved in ribosome biogenesis induces chromosomal instability. | Killian, A., et al. 2004. Oncogene. 23: 8597-602. PMID: 15467761
- Human pescadillo induces large-scale chromatin unfolding. | Zhang, H., et al. 2005. Sci China C Life Sci. 48: 270-6. PMID: 16092760
- Pescadillo interacts with the cadmium response element of the human heme oxygenase-1 promoter in renal epithelial cells. | Sikorski, EM., et al. 2006. J Biol Chem. 281: 24423-30. PMID: 16816389
- Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. | Allende, ML., et al. 1996. Genes Dev. 10: 3141-55. PMID: 8985183
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
pescadillo Antibody (H-10) | sc-166300 | 200 µg/ml | $322.00 | |||
pescadillo Antibody (H-10): m-IgG Fc BP-HRP Bundle | sc-528939 | 200 µg Ab; 10 µg BP | $361.00 | |||
pescadillo Antibody (H-10): m-IgGκ BP-HRP Bundle | sc-521561 | 200 µg Ab, 40 µg BP | $361.00 | |||
pescadillo Antibody (H-10): m-IgG1 BP-HRP Bundle | sc-543224 | 200 µg Ab; 20 µg BP | $361.00 | |||
pescadillo Antibody (H-10) X | sc-166300 X | 200 µg/0.1 ml | $322.00 | |||
pescadillo Antibody (H-10) AC | sc-166300 AC | 500 µg/ml, 25% agarose | $424.00 | |||
pescadillo Antibody (H-10) HRP | sc-166300 HRP | 200 µg/ml | $322.00 | |||
pescadillo Antibody (H-10) FITC | sc-166300 FITC | 200 µg/ml | $336.00 | |||
pescadillo Antibody (H-10) PE | sc-166300 PE | 200 µg/ml | $349.00 | |||
pescadillo Antibody (H-10) Alexa Fluor® 488 | sc-166300 AF488 | 200 µg/ml | $364.00 | |||
pescadillo Antibody (H-10) Alexa Fluor® 546 | sc-166300 AF546 | 200 µg/ml | $364.00 | |||
pescadillo Antibody (H-10) Alexa Fluor® 594 | sc-166300 AF594 | 200 µg/ml | $364.00 | |||
pescadillo Antibody (H-10) Alexa Fluor® 647 | sc-166300 AF647 | 200 µg/ml | $364.00 | |||
pescadillo Antibody (H-10) Alexa Fluor® 680 | sc-166300 AF680 | 200 µg/ml | $364.00 | |||
pescadillo Antibody (H-10) Alexa Fluor® 790 | sc-166300 AF790 | 200 µg/ml | $364.00 | |||
pescadillo (H-10) Neutralizing Peptide | sc-166300 P | 100 µg/0.5 ml | $69.00 |