Peroxin 5R Antibody (A-4): sc-398580

Peroxin 5R Antibody (A-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects Peroxin 5R in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Peroxin 5R (A-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Peroxin 5R, also known as PEX5-related protein or Peroxisome biogenesis factor 5-like, is a crucial component of peroxisome biogenesis, a process essential for the proper functioning of peroxisomes—organelles that play a vital role in lipid metabolism and the detoxification of hydrogen peroxide. This 626 amino acid protein is predominantly expressed in the brain, with additional expression in the testis and pancreas, and features five tetratricopeptide repeats that facilitate protein-protein interactions and the assembly of multiprotein complexes. Notably, Peroxin 5R interacts with Rab 8b, suggesting involvement in vesicular trafficking and neurotransmitter release, which are critical for maintaining cellular homeostasis and signaling. The presence of three isoforms of Peroxin 5R, generated through alternative splicing, allows for diverse functional roles in cellular processes, particularly in recognizing C-terminal peroxisome-targeting signals akin to those recognized by Peroxin-5.

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Peroxin 5R Antibody (A-4) References:

1. The mammalian peroxin Pex5pL, the longer isoform of the mobile peroxisome targeting signal (PTS) type 1 transporter, translocates the Pex7p.PTS2 protein complex into peroxisomes via its initial docking site, Pex14p.  |  Otera, H., et al. 2000. J Biol Chem. 275: 21703-14. PMID: 10767286
2. Disruption of the interaction of the longer isoform of Pex5p, Pex5pL, with Pex7p abolishes peroxisome targeting signal type 2 protein import in mammals. Study with a novel Pex5-impaired Chinese hamster ovary cell mutant.  |  Matsumura, T., et al. 2000. J Biol Chem. 275: 21715-21. PMID: 10767287
3. Peroxisome biogenesis and peroxisome biogenesis disorders.  |  Fujiki, Y. 2000. FEBS Lett. 476: 42-6. PMID: 10878247
4. Identification of PEX5p-related novel peroxisome-targeting signal 1 (PTS1)-binding proteins in mammals.  |  Amery, L., et al. 2001. Biochem J. 357: 635-46. PMID: 11463335
5. Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import.  |  Otera, H., et al. 2002. Mol Cell Biol. 22: 1639-55. PMID: 11865044
6. Pex5p binding affinities for canonical and noncanonical PTS1 peptides.  |  Maynard, EL., et al. 2004. Proteins. 55: 856-61. PMID: 15146484
7. Hidden localization motifs: naturally occurring peroxisomal targeting signals in non-peroxisomal proteins.  |  Neuberger, G., et al. 2004. Genome Biol. 5: R97. PMID: 15575971
8. Peroxin 5-peroxin 14 association in the protozoan Leishmania donovani involves a novel protein-protein interaction motif.  |  Madrid, KP. and Jardim, A. 2005. Biochem J. 391: 105-14. PMID: 15929724
9. Molecular mechanisms of import of peroxisome-targeting signal type 2 (PTS2) proteins by PTS2 receptor Pex7p and PTS1 receptor Pex5pL.  |  Mukai, S. and Fujiki, Y. 2006. J Biol Chem. 281: 37311-20. PMID: 17040904
10. Genetic variants in DDO and PEX5L in peroxisome-related pathways predict non-small cell lung cancer survival.  |  Chen, AS., et al. 2022. Mol Carcinog. 61: 619-628. PMID: 35502931
11. Dynamics of the translocation pore of the human peroxisomal protein import machinery.  |  Ghosh, M., et al. 2023. Biol Chem. 404: 169-178. PMID: 35977096
12. Membrane binding and pore forming insertion of PEX5 into horizontal lipid bilayer.  |  Blum, D., et al. 2023. Biol Chem. 404: 157-167. PMID: 36260915

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Peroxin 5R Antibody (A-4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Peroxin 5R Antibody (A-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Peroxin 5R Antibody (A-4) Specifications

Alternate Product Name: Peroxin 5R

Product Clone Name: A-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 161-176

Epitope Target: Peroxin 5R

Epitope Species: human

Product Reactivity/Detection: Peroxin 5R

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Peroxin 5R Antibody (A-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin