periaxin CRISPR/Cas9 KO Plasmid (h): sc-404868
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- periaxin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the periaxin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: periaxin Antibody (G-5): sc-515672
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
periaxin CRISPR/Cas9 KO Plasmid (h) | sc-404868 | 20 µg | $397.00 |
Overview
PRX encodes periaxin, a Schwann cell–enriched scaffolding protein that is essential for peripheral nerve myelin stability and maintenance. Periaxin participates in cytoskeletal organization and membrane domain architecture, supporting Schwann cell elongation and the formation of myelin-associated complexes that govern axon–glia interactions. Disruption of PRX perturbs myelin homeostasis and nerve conduction, linking altered periaxin function to inherited peripheral neuropathies, including Charcot–Marie–Tooth disease type 4F and Dejerine–Sottas–like phenotypes. Consequently, PRX is widely studied in pathways controlling myelination, Schwann cell differentiation, and stress responses that influence peripheral nerve integrity.
periaxin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRX gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRX together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRX open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish periaxin protein expression.
This CRISPR knockout system enables efficient generation of PRX-deficient cell models for investigation of periaxin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRX exon(s) critical for periaxin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRX genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by periaxin CRISPR/Cas9 KO Plasmid (h) and periaxin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRX locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by periaxin HDR Plasmid (h) and periaxin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRX homology arms to support homology-directed repair at defined PRX target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.