Perforin 1 Antibody (A-2): sc-373943

Perforin 1 Antibody (A-2) is a mouse monoclonal IgG1 antibody that detects perforin 1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Perforin 1 plays a crucial role in the immune response by facilitating the destruction of virus-infected and tumorigenic cells through cytotoxic T lymphocyte (CTL)-mediated cytotoxicity. Perforin 1 is primarily located in cytotoxic T lymphocytes and natural killer cells, where perforin 1 is secreted in response to immune activation. The presence of perforin 1 in these immune cells is vital, as perforin 1 allows for the formation of pores in the membranes of target cells, enabling the entry of cytotoxic serine proteases known as granzymes, which induce apoptosis. Additionally, perforin 1 has been identified in a specific population of astrocytes in the human brain, suggesting a potential role in neuroimmune interactions. The activity of perforin 1 is regulated by various cytokines, including interleukin-2, interleukin-3, interleukin-4, and interleukin-6, highlighting perforin 1′s importance in both immune defense and the modulation of inflammatory responses. Perforin 1 (A-2) antibody is an essential tool for researchers studying immune responses, cell-mediated cytotoxicity, and the underlying mechanisms of autoimmune diseases and transplant rejection.

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Perforin 1 Antibody (A-2) References:

1. MicroRNA-150 regulates the cytotoxicity of natural killers by targeting perforin-1.  |  Kim, N., et al. 2014. J Allergy Clin Immunol. 134: 195-203. PMID: 24698324
2. Late-onset severe chronic active EBV in a patient for five years with mutations in STXBP2 (MUNC18-2) and PRF1 (perforin 1).  |  Cohen, JI., et al. 2015. J Clin Immunol. 35: 445-8. PMID: 25947952
3. Spectrum of Atypical Clinical Presentations in Patients with Biallelic PRF1 Missense Mutations.  |  Tesi, B., et al. 2015. Pediatr Blood Cancer. 62: 2094-100. PMID: 26184781
4. Missense mutations in the perforin (PRF1) gene as a cause of hereditary cancer predisposition.  |  Chaudhry, MS., et al. 2016. Oncoimmunology. 5: e1179415. PMID: 27622035
5. Rare cause of Hemophagocytic Lymphohistiocytosis due to mutation in PRF1 and SH2D1A genes in two children - a case report with a review.  |  Sheth, J., et al. 2019. BMC Pediatr. 19: 73. PMID: 30849948
6. Structure, function and expression of murine and human perforin 1 (P1).  |  Podack, ER., et al. 1988. Immunol Rev. 103: 203-11. PMID: 3292394
7. PRF1 mutation alters immune system activation, inflammation, and risk of autoimmunity.  |  Sidore, C., et al. 2021. Mult Scler. 27: 1332-1340. PMID: 33566725
8. PRF1 is a prognostic marker and correlated with immune infiltration in head and neck squamous cell carcinoma.  |  Fan, C., et al. 2021. Transl Oncol. 14: 101042. PMID: 33618069
9. Late Onset of Primary Hemophagocytic Lymphohistiocytosis (HLH) with a Novel Constellation of Compound Heterozygosity Involving Two Missense Variants in the PRF1 Gene.  |  Stadermann, A., et al. 2024. Int J Mol Sci. 25: PMID: 38474010
10. Disruption of IFNγ, GZMB, PRF1, or LYST Results in Reduced Suppressive Function in Human CD8+ T Cells.  |  Vemulawada, C., et al. 2024. J Immunol. 212: 1722-1732. PMID: 38607279

The preferred Perforin 1 antibody is Perforin 1 Antibody (F-1): sc-136994.