Per1 CRISPR/Cas9 KO Plasmid (h): sc-401260
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Per1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Per1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Per1 Antibody (E-8): sc-398890
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Per1 CRISPR/Cas9 KO Plasmid (h) | sc-401260 | 20 µg | $397.00 |
Overview
PER1 encodes the Period 1 protein, a core component of the molecular circadian clock that participates in transcription–translation feedback loops controlling 24-hour rhythmic gene expression. PER1 interacts with other clock regulators to modulate CLOCK/ARNTL (BMAL1)-driven transcription, linking circadian timing to cell cycle control, DNA damage responses, metabolism, and hormone signaling. Through these networks, PER1 influences temporal coordination of cellular stress pathways and mitochondrial and redox homeostasis. Dysregulation of PER1 expression or rhythmicity has been associated with altered sleep–wake physiology and has been reported in studies of cancer biology, metabolic disorders, and neuropsychiatric disease mechanisms.
Per1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PER1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PER1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PER1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Per1 protein expression.
This CRISPR knockout system enables efficient generation of PER1-deficient cell models for investigation of Per1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PER1 exon(s) critical for Per1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PER1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Per1 CRISPR/Cas9 KO Plasmid (h) and Per1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PER1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Per1 HDR Plasmid (h) and Per1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PER1 homology arms to support homology-directed repair at defined PER1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.