Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Pepsin C CRISPR/Cas9 KO Plasmid (h): sc-404137
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pepsin C CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pepsin C genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pepsin C Antibody (E-9): sc-374044
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pepsin C CRISPR/Cas9 KO Plasmid (h) | sc-404137 | 20 µg | $397.00 |
Overview
PGC encodes pepsin C, an aspartic endopeptidase (gastricsin) secreted as a zymogen and activated under acidic conditions to participate in gastric proteolysis. In the stomach, pepsin C contributes to luminal protein digestion and peptide processing, functioning within acid-dependent protease networks that complement pepsin A activity. Altered PGC expression and pepsin C secretion patterns have been used as indicators of changes in gastric mucosal differentiation and epithelial homeostasis. Dysregulation of gastric protease programs is frequently examined in the context of inflammatory remodeling and neoplastic transformation of the upper gastrointestinal tract, supporting mechanistic studies in gastric cell models and tissue-derived systems.
Pepsin C CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PGC gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PGC together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PGC open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pepsin C protein expression.
This CRISPR knockout system enables efficient generation of PGC-deficient cell models for investigation of Pepsin C signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PGC exon(s) critical for Pepsin C function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PGC genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pepsin C CRISPR/Cas9 KO Plasmid (h) and Pepsin C CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PGC locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pepsin C HDR Plasmid (h) and Pepsin C HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PGC homology arms to support homology-directed repair at defined PGC target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.