Pepsin A Antibody (D-5): sc-365680

Pepsin A Antibody (D-5) is a mouse monoclonal IgG2a antibody that detects pepsin A in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Pepsin A plays a crucial role in the digestive system as one of the primary proteolytic enzymes secreted by the gastric mucosa, where pepsin A is responsible for breaking down proteins into smaller peptides, facilitating nutrient absorption. The enzyme is synthesized as an inactive precursor, pepsinogen, which is activated in the highly acidic environment of the stomach (pH 1-3) through the action of hydrochloric acid. This activation process is vital, as pepsin A is only active in the stomach, preventing potential damage to the gastric lining and allowing for efficient protein digestion. Pepsin A exhibits broad specificity, cleaving peptide bonds involving aromatic amino acids, with a preference for phenylalanine and leucine, which is essential for the effective breakdown of dietary proteins. Additionally, pepsin A is classified as a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase found in vertebrate gastric juice, highlighting pepsin A′s importance in digestive physiology. Anti-pepsin A antibody (D-5) is an invaluable tool for researchers studying digestive enzymes and their roles in human health and disease.

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Pepsin A Antibody (D-5) References:

1. Gastric antibacterial efficiency is different for pepsin A and C.  |  Schreiber, S., et al. 2006. Arch Microbiol. 184: 335-40. PMID: 16333616
2. Reduced pepsin A processing of sonic hedgehog in parietal cells precedes gastric atrophy and transformation.  |  Zavros, Y., et al. 2007. J Biol Chem. 282: 33265-33274. PMID: 17872943
3. Pepsin, a marker of gastric contents, is increased in tracheal aspirates from preterm infants who develop bronchopulmonary dysplasia.  |  Farhath, S., et al. 2008. Pediatrics. 121: e253-9. PMID: 18245400
4. Differences in the P1' substrate specificities of pepsin A and chymosin.  |  Kageyama, H., et al. 2010. J Biochem. 147: 167-74. PMID: 19819898
5. Effects of pepsin A on heat shock protein 70 response in laryngopharyngeal reflux patients with chronic rhinosinusitis.  |  Wang, J., et al. 2017. Acta Otolaryngol. 137: 1253-1259. PMID: 28784015
6. Salivary pepsin A detection related to gastro-oesophageal reflux episodes in children undergoing impedance probe monitoring.  |  Haddad, HA., et al. 2020. Acta Paediatr. 109: 2374-2379. PMID: 32239539
7. The Controversy of Pepsinogen A/Pepsin A in Detecting Extra-Gastroesophageal Reflux.  |  Rao, YF., et al. 2023. J Voice. 37: 748-756. PMID: 34090740
8. Pepsin A in Tracheal Secretions From Patients Receiving Mechanical Ventilation.  |  Talbert, S., et al. 2021. Am J Crit Care. 30: 443-450. PMID: 34719715
9. Use of Machine Learning Models to Predict Microaspiration Measured by Tracheal Pepsin A.  |  Bourgault, A., et al. 2025. Am J Crit Care. 34: 67-71. PMID: 39740967
10. A comparative study on amino acid sequences of three major isoforms of human pepsin A.  |  Tarasova, N., et al. 1995. Adv Exp Med Biol. 362: 77-81. PMID: 8540383

The preferred Pepsin A antibody is Pepsin A Antibody (A-10): sc-271798.