Pellino 1/2 Antibody (F-7): sc-271065

Pellino 1/2 Antibody (F-7) is a mouse monoclonal IgG2a kappa light chain antibody that detects Pellino 1/2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Pellino 1/2 (F-7) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Pellino proteins, including Pellino 1 and 2, play a crucial role as scaffolding components in Toll-like receptor (TLR) and Interleukin-1 (IL-1) receptor signaling pathways, which are essential for the immune response. Specifically, Pellino 2 initiates mitogen-activated protein kinase (MAPK) pathways, leading to the activation of transcription factors such as AP-1 and Elk-1, which are vital for regulating gene expression in response to inflammatory signals. The ability of Pellino 2 to activate these pathways underscores its importance in mediating cellular responses to stress and inflammation, making Pellino 2 a significant target for research in immunology and related fields. Pellino 1/2 (F-7) monoclonal antibody serves as an invaluable tool for studying these critical signaling mechanisms in various biological contexts.

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Pellino 1/2 Antibody (F-7) References:

1. Pellino-related sequences from Caenorhabditis elegans and Homo sapiens.  |  Rich, T., et al. 2000. Immunogenetics. 52: 145-9. PMID: 11132151
2. Assignment of homologous genes, Peli1/PELI1 and Peli2/PELI2, for the Pelle adaptor protein Pellino to mouse chromosomes 11 and 14 and human chromosomes 2p13.3 and 14q21, respectively, by physical and radiation hybrid mapping.  |  Resch, K., et al. 2001. Cytogenet Cell Genet. 92: 172-4. PMID: 11306823
3. Cutting edge: mouse pellino-2 modulates IL-1 and lipopolysaccharide signaling.  |  Yu, KY., et al. 2002. J Immunol. 169: 4075-8. PMID: 12370331
4. Pellino 1 is required for interleukin-1 (IL-1)-mediated signaling through its interaction with the IL-1 receptor-associated kinase 4 (IRAK4)-IRAK-tumor necrosis factor receptor-associated factor 6 (TRAF6) complex.  |  Jiang, Z., et al. 2003. J Biol Chem. 278: 10952-6. PMID: 12496252
5. Pellino2 activates the mitogen activated protein kinase pathway.  |  Jensen, LE. and Whitehead, AS. 2003. FEBS Lett. 545: 199-202. PMID: 12804775
6. Pellino3, a novel member of the Pellino protein family, promotes activation of c-Jun and Elk-1 and may act as a scaffolding protein.  |  Jensen, LE. and Whitehead, AS. 2003. J Immunol. 171: 1500-6. PMID: 12874243
7. Pellino3 is a novel upstream regulator of p38 MAPK and activates CREB in a p38-dependent manner.  |  Butler, MP., et al. 2005. J Biol Chem. 280: 27759-68. PMID: 15917247
8. Pellino 2 is critical for Toll-like receptor/interleukin-1 receptor (TLR/IL-1R)-mediated post-transcriptional control.  |  Kim, TW., et al. 2012. J Biol Chem. 287: 25686-95. PMID: 22669975
9. Pellino-1 Protects Periodontal Ligament Stem Cells Against H2O2-Induced Apoptosis via Activation of NF-κB Signaling.  |  Tian, J., et al. 2018. Mol Biotechnol. 60: 533-538. PMID: 29860626
10. Gene therapy with Pellino-1 improves perfusion and decreases tissue loss in Flk-1 heterozygous mice but fails in MAPKAP Kinase-2 knockout murine hind limb ischemia model.  |  Thirunavukkarasu, M., et al. 2022. Microvasc Res. 141: 104311. PMID: 34999110
11. Pellino-1 promotes intrinsic activation of skin-resident IL-17A-producing T cells in psoriasis.  |  Kim, SH., et al. 2023. J Allergy Clin Immunol. 151: 1317-1328. PMID: 36646143
12. Pellino-1, a therapeutic target for control of SARS-CoV-2 infection and disease severity.  |  Wang, B., et al. 2025. Antiviral Res. 233: 106059. PMID: 39689784

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pellino 1/2 Antibody (F-7) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-Pellino 1/2 Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pellino 1/2 Antibody (F-7) Specifications

Alternate Product Name: Pellino 1/2

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 151-255

Epitope Target: Pellino 1

Epitope Species: human

Product Reactivity/Detection: Pellino 1 and Pellino 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine and porcine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for Pellino 1/2 Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin