CD31/PECAM-1 Antibody (158-2B3): sc-65260

PECAM-1 Antibody (158-2B3) is a mouse monoclonal IgG1 kappa light chain antibody that detects human PECAM-1 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-PECAM-1 antibody (158-2B3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PECAM-1, also known as CD31, is a crucial glycoprotein expressed on the surfaces of monocytes, neutrophils, platelets, and a subset of T cells, playing a significant role in mediating cell-cell adhesion and signaling during immune responses and inflammation. PECAM-1 is primarily located at the intercellular junctions of endothelial cells, where PECAM-1 facilitates the migration of leukocytes across the vascular endothelium, a process essential for immune surveillance and tissue repair. Understanding PECAM-1 localization and function is vital, as PECAM-1 dysregulation can contribute to various pathological conditions, including chronic inflammation and cancer metastasis. PECAM-1 interaction with other adhesion molecules, such as integrins, further underscores PECAM-1′s importance in maintaining vascular integrity and regulating immune cell trafficking.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD31/PECAM-1 Antibody (158-2B3) References:

1. PECAM-1 dampens cytokine levels during LPS-induced endotoxemia by regulating leukocyte trafficking.  |  Privratsky, JR., et al. 2012. Life Sci. 90: 177-84. PMID: 22119535
2. PECAM-1 isoforms, eNOS and endoglin axis in regulation of angiogenesis.  |  Park, S., et al. 2015. Clin Sci (Lond). 129: 217-34. PMID: 25976664
3. The adhesion molecule PECAM-1 enhances the TGF-β-mediated inhibition of T cell function.  |  Newman, DK., et al. 2016. Sci Signal. 9: ra27. PMID: 26956486
4. Roles of PECAM-1 in cell function and disease progression.  |  Feng, YM., et al. 2016. Eur Rev Med Pharmacol Sci. 20: 4082-4088. PMID: 27775789
5. Influence of PECAM-1 ligand interactions on PECAM-1-dependent cell motility and filopodia extension.  |  Abraham, V., et al. 2016. Physiol Rep. 4: PMID: 27895229
6. Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination.  |  Abraham, V., et al. 2018. Int J Oncol. 53: 488-502. PMID: 29845213
7. PECAM-1 directed re-targeting of exogenous mRNA providing two orders of magnitude enhancement of vascular delivery and expression in lungs independent of apolipoprotein E-mediated uptake.  |  Parhiz, H., et al. 2018. J Control Release. 291: 106-115. PMID: 30336167
8. Heparan sulfate proteoglycan glypican-1 and PECAM-1 cooperate in shear-induced endothelial nitric oxide production.  |  Bartosch, AMW., et al. 2021. Sci Rep. 11: 11386. PMID: 34059731
9. PECAM-1 drives β-catenin-mediated EndMT via internalization in colon cancer with diabetes mellitus.  |  Wu, Q., et al. 2023. Cell Commun Signal. 21: 203. PMID: 37580771
10. LAIR-1 and PECAM-1 function via the same signaling pathway to inhibit GPVI-mediated platelet activation.  |  Smith, CW., et al. 2024. Res Pract Thromb Haemost. 8: 102557. PMID: 39318773

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD31/PECAM-1 Antibody (158-2B3) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CD31/PECAM-1 Antibody (158-2B3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD31/PECAM-1 Antibody (158-2B3) Specifications

Alternate Product Name: CD31

Product Clone Name: 158-2B3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: PECAM-1

Epitope Species: human

Product Reactivity/Detection: PECAM-1

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CD31/PECAM-1 Antibody (158-2B3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD31/PECAM-1 antibody is CD31/PECAM-1 Antibody (MEC 13.3): sc-18916.