Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PEBP2β CRISPR/Cas9 KO Plasmid (h): sc-401983
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PEBP2β CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PEBP2β genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PEBP2β Antibody (141,4,1): sc-56751
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PEBP2β CRISPR/Cas9 KO Plasmid (h) | sc-401983 | 20 µg | $397.00 |
Overview
CBFB encodes the core-binding factor subunit beta (PEBP2β), a non–DNA-binding partner that heterodimerizes with RUNX transcription factors to stabilize DNA binding and coordinate gene expression programs controlling hematopoietic differentiation, osteogenesis, and immune cell development. Through this RUNX/CBF complex, CBFB helps regulate pathways governing cell fate commitment, proliferation, and lineage-specific transcriptional networks. Disruption of CBFB-dependent transcription is implicated in aberrant hematopoiesis and developmental defects, and CBFB is frequently studied in the context of chromosomal rearrangements and transcriptional dysregulation in hematologic disease models. These properties make CBFB a key node for investigating transcription factor cooperativity, enhancer regulation, and lineage specification mechanisms.
PEBP2β CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CBFB gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CBFB together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CBFB open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PEBP2β protein expression.
This CRISPR knockout system enables efficient generation of CBFB-deficient cell models for investigation of PEBP2β signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CBFB exon(s) critical for PEBP2β function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CBFB genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PEBP2β CRISPR/Cas9 KO Plasmid (h) and PEBP2β CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CBFB locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PEBP2β HDR Plasmid (h) and PEBP2β HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CBFB homology arms to support homology-directed repair at defined CBFB target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.