PDX-1 Antibody (E-8): sc-398502
- PDX-1 Antibody (E-8) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 95-110 within an internal region of PDX-1 of human origin
- recommended for detection of PDX-1 of mouse, rat and human origin by WB, IP, IF and ELISA
- TransCruz reagent for ChIP application (sc-398502 X, 200 µg/0.1 ml)
- See PDX-1 (B-11): sc-390792 for PDX-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PDX-1 Antibody (E-8). This work is in progress.
QUICK LINKS
PDX-1 (E-8) is a mouse monoclonal IgM antibody. Pancreatic duodenal homeobox-1 protein (PDX-1), also designated insulin promoter factor (IPF1), insulin upstream factor 1 (IUF1), somatostatin trans-activating factor-1 (STF-1) and glucose-sensitive factor (GSF), is a 282 amino acid homeodomain-containing transcription factor present in pancreatic beta-cells. PDX-1 is a key regulator of pancreatic islet development and insulin gene transcription in beta-cells. PDX-1 is expressed in all cells at early stages of development and is mainly restricted to pancreas and duodenum in adult. HNF-3b, HNF-1a and SP1 positively regulate PDX-1 enhancer element. PDX-1 is also regulated by glucagon-like peptide through activation of adenylyl cyclase, which results in an increase in intracellular cAMP activity. Increased levels of cAMP, and resulting activation of PKA, lead to an increase in PDX-1 transcription and translocation to beta-cell nuclei. PDX-1 binds to sequence C(C/T) and can heterodimerize with PBX. PDX-1 is phosphorylated by SAPK2 pathway under high glucose concentrations. Mutations in PDX-1 gene can cause maturity-onset diabetes of young and pancreatic agenesis. PDX-1 gene maps to human chromosome 13q12.1.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDX-1 Antibody (E-8) References:
- Pancreatic Duodenal Homeobox (PDX-1) in health and disease. | Melloul, D., et al. 2002. J Pediatr Endocrinol Metab. 15: 1461-72. PMID: 12503852
- PDX-1/VP16 fusion protein, together with NeuroD or Ngn3, markedly induces insulin gene transcription and ameliorates glucose tolerance. | Kaneto, H., et al. 2005. Diabetes. 54: 1009-22. PMID: 15793239
- Adenovirus transduction is required for the correction of diabetes using Pdx-1 or Neurogenin-3 in the liver. | Wang, AY., et al. 2007. Mol Ther. 15: 255-63. PMID: 17235302
- PDX-1 functions as a master factor in the pancreas. | Kaneto, H., et al. 2008. Front Biosci. 13: 6406-20. PMID: 18508668
- Metformin Restrains Pancreatic Duodenal Homeobox-1 (PDX-1) Function by Inhibiting ERK Signaling in Pancreatic Ductal Adenocarcinoma. | Zhou, G., et al. 2016. Curr Mol Med. 16: 83-90. PMID: 26695692
- Impact of Pdx1-associated chromatin modifiers on islet β-cells. | Spaeth, JM., et al. 2016. Diabetes Obes Metab. 18 Suppl 1: 123-7. PMID: 27615141
- Novel codons in rat Pdx-1 complementary DNA. | Kiba, T. 2018. BMC Res Notes. 11: 726. PMID: 30314444
- Pdx1 Is Transcriptionally Regulated by EGR-1 during Nitric Oxide-Induced Endoderm Differentiation of Mouse Embryonic Stem Cells. | Salguero-Aranda, C., et al. 2022. Int J Mol Sci. 23: PMID: 35409280
- Biophysical insights into glucose-dependent transcriptional regulation by PDX1. | Usher, ET. and Showalter, SA. 2022. J Biol Chem. 298: 102623. PMID: 36272648
- PDX1 is the cornerstone of pancreatic β-cell functions and identity. | Ebrahim, N., et al. 2022. Front Mol Biosci. 9: 1091757. PMID: 36589234
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDX-1 Antibody (E-8) | sc-398502 | 200 µg/ml | $322.00 | |||
PDX-1 Antibody (E-8): m-IgGκ BP-HRP Bundle | sc-551785 | 200 µg Ab; 40 µg BP | $361.00 | |||
PDX-1 (E-8) Neutralizing Peptide | sc-398502 P | 100 µg/0.5 ml | $69.00 | |||
PDX-1 Antibody (E-8) X | sc-398502 X | 200 µg/0.1 ml | $322.00 |