PDK2 Antibody (S-15): sc-100534

PDK2 Antibody (S-15) is a mouse monoclonal IgG2a kappa light chain antibody that detects PDK2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-PDK2 antibody (S-15) is available as the non-conjugated form. PDK2, a member of the pyruvate dehydrogenase kinase family, plays a crucial role in cellular metabolism by regulating the activity of the pyruvate dehydrogenase complex (PDC) through phosphorylation of its E1α subunit. This regulation is vital as it determines whether pyruvate is converted into acetyl-CoA for energy production or diverted towards other metabolic pathways, particularly during periods of nutrient deprivation. The PDC is composed of multiple dihydrolipoamide acetyltransferase (E2) subunits, which interact with PDK2 to enhance its kinase activity, thereby influencing metabolic flexibility. Notably, PDK2 expression is upregulated under starvation conditions, promoting fatty acid oxidation and altering acetyl-CoA generation to meet the energy demands of the cell. PDK2′s structure includes five conserved regions that are essential for kinase activity, and PDK2 shares mechanistic similarities with bacterial His-kinases, highlighting evolutionary significance. PDK2 transcripts are widely expressed across various tissues, with particularly high levels found in heart and skeletal muscle, underscoring importance in energy metabolism in these tissues.

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PDK2 Antibody (S-15) References:

1. Histidine modifying agents abolish pyruvate dehydrogenase kinase activity.  |  Mooney, BP., et al. 2000. Biochem Biophys Res Commun. 267: 500-3. PMID: 10631090
2. Marked differences between two isoforms of human pyruvate dehydrogenase kinase.  |  Baker, JC., et al. 2000. J Biol Chem. 275: 15773-81. PMID: 10748134
3. Starvation increases the amount of pyruvate dehydrogenase kinase in several mammalian tissues.  |  Wu, P., et al. 2000. Arch Biochem Biophys. 381: 1-7. PMID: 11019813
4. Selective modification of the pyruvate dehydrogenase kinase isoform profile in skeletal muscle in hyperthyroidism: implications for the regulatory impact of glucose on fatty acid oxidation.  |  Sugden, MC., et al. 2000. J Endocrinol. 167: 339-45. PMID: 11054649
5. PDK2-enhanced glycolysis promotes fibroblast proliferation in thyroid-associated ophthalmopathy.  |  Ma, R., et al. 2020. J Mol Endocrinol. 65: 163-174. PMID: 33086191
6. PDK2 Deficiency Prevents Ovariectomy-Induced Bone Loss in Mice by Regulating the RANKL-NFATc1 Pathway During Osteoclastogenesis.  |  Lee, JM., et al. 2021. J Bone Miner Res. 36: 553-566. PMID: 33125772
7. DCA Protects against Oxidation Injury Attributed to Cerebral Ischemia-Reperfusion by Regulating Glycolysis through PDK2-PDH-Nrf2 Axis.  |  Zhao, X., et al. 2021. Oxid Med Cell Longev. 2021: 5173035. PMID: 34712383
8. Targeting PDK2 rescues stress-induced impaired brain energy metabolism.  |  Wang, C., et al. 2023. Mol Psychiatry. 28: 4138-4150. PMID: 37188779
9. The OGT-c-Myc-PDK2 axis rewires the TCA cycle and promotes colorectal tumor growth.  |  Wang, H., et al. 2024. Cell Death Differ. 31: 1157-1169. PMID: 38778217
10. Cordycepin Modulates Microglial M2 Polarization Coupled with Mitochondrial Metabolic Reprogramming by Targeting HKII and PDK2.  |  Zhong, X., et al. 2024. Adv Sci (Weinh). 11: e2304687. PMID: 38889331
11. Diversity of the pyruvate dehydrogenase kinase gene family in humans.  |  Gudi, R., et al. 1995. J Biol Chem. 270: 28989-94. PMID: 7499431
12. Evidence for existence of tissue-specific regulation of the mammalian pyruvate dehydrogenase complex.  |  Bowker-Kinley, MM., et al. 1998. Biochem J. 329 (Pt 1): 191-6. PMID: 9405293

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDK2 Antibody (S-15) has 10 citations in a variety of scientific publications.

Primary antibodies like Anti-PDK2 Antibody (S-15) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDK2 Antibody (S-15) Specifications

Alternate Product Name: PDK2

Product Clone Name: S-15

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 187-276

Epitope Target: PDK2

Epitope Species: human

Product Reactivity/Detection: PDK2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 10 citations in various scientific publications

Available Conjugates/Forms for PDK2 Antibody (S-15): N/A