Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PDGF-A CRISPR/Cas9 KO Plasmid (h): sc-400711
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PDGF-A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PDGF-A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PDGF-A Antibody (E-10): sc-9974
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDGF-A CRISPR/Cas9 KO Plasmid (h) | sc-400711 | 20 µg | $397.00 |
Overview
PDGFA encodes platelet-derived growth factor A (PDGF-A), a secreted dimeric growth factor that signals primarily through PDGFRα to regulate mesenchymal cell proliferation, migration, and survival. PDGF-A is a key driver of mitogenic and chemotactic responses that interface with MAPK/ERK and PI3K/AKT signaling, contributing to extracellular matrix remodeling, angiogenic support, and stromal–epithelial crosstalk. Dysregulated PDGF-A/PDGFR signaling has been implicated in fibrotic remodeling and tumor-associated stromal activation, and it is frequently studied for its effects on cell cycle control and tissue microenvironment dynamics. PDGFA is also relevant to developmental processes and wound repair models where paracrine growth factor gradients shape cell fate and motility.
PDGF-A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PDGFA gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PDGFA together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PDGFA open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PDGF-A protein expression.
This CRISPR knockout system enables efficient generation of PDGFA-deficient cell models for investigation of PDGF-A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PDGFA exon(s) critical for PDGF-A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PDGFA genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PDGF-A CRISPR/Cas9 KO Plasmid (h) and PDGF-A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PDGFA locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PDGF-A HDR Plasmid (h) and PDGF-A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PDGFA homology arms to support homology-directed repair at defined PDGFA target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.