PDEF Antibody (G-10): sc-166846

PDEF Antibody (G-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDEF protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDEF (G-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The Prostate Epithelium-Specific Ets Transcription Factor (PDEF), also known as prostate Ets or SAM pointed domain containing Ets transcription factor, is a 335 amino acid nuclear protein that plays a crucial role in the regulation of gene expression within prostate epithelial cells. As a member of the Ets family of transcription factors, PDEF is primarily located in the nucleus, where PDEF is essential for modulating the transcriptional activity of genes involved in prostate development and function. Notably, PDEF upregulates the activity of the p62 promoter, although this activity can be inhibited by the proteasome inhibitor PSI. Additionally, PDEF acts as an androgen-independent transactivator of prostate-specific antigen (PSA), highlighting PDEF′s significance in prostate cancer biology and potential as a therapeutic target.

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PDEF Antibody (G-10) References:

1. PDEF, a novel prostate epithelium-specific ets transcription factor, interacts with the androgen receptor and activates prostate-specific antigen gene expression.  |  Oettgen, P., et al. 2000. J Biol Chem. 275: 1216-25. PMID: 10625666
2. Cloning and expression of the mouse Pse gene encoding a novel Ets family member.  |  Yamada, N., et al. 2000. Gene. 241: 267-74. PMID: 10675039
3. NKX-3.1 interacts with prostate-derived Ets factor and regulates the activity of the PSA promoter.  |  Chen, H., et al. 2002. Cancer Res. 62: 338-40. PMID: 11809674
4. p62 overexpression in breast tumors and regulation by prostate-derived Ets factor in breast cancer cells.  |  Thompson, HG., et al. 2003. Oncogene. 22: 2322-33. PMID: 12700667
5. Pdef expression in human breast cancer is correlated with invasive potential and altered gene expression.  |  Feldman, RJ., et al. 2003. Cancer Res. 63: 4626-31. PMID: 12907642
6. Prostate derived Ets transcription factor shows better tumor-association than other cancer-associated molecules.  |  Ghadersohi, A., et al. 2004. Oncol Rep. 11: 453-8. PMID: 14719083
7. Structural and functional analysis of domains mediating interaction between NKX-3.1 and PDEF.  |  Chen, H. and Bieberich, CJ. 2005. J Cell Biochem. 94: 168-77. PMID: 15523673
8. Analysis of the 2.0 A crystal structure of the protein-DNA complex of the human PDEF Ets domain bound to the prostate specific antigen regulatory site.  |  Wang, Y., et al. 2005. Biochemistry. 44: 7095-106. PMID: 15882048
9. Prostate-derived Ets transcription factor (PDEF) is a potential prognostic marker in patients with prostate cancer.  |  Ghadersohi, A., et al. 2011. Prostate. 71: 1178-88. PMID: 21656828
10. PDEF downregulates stathmin expression in prostate cancer.  |  Sabherwal, Y., et al. 2012. Int J Oncol. 40: 1889-99. PMID: 22378487
11. Prostate derived Ets transcription factor and Carcinoembryonic antigen related cell adhesion molecule 6 constitute a highly active oncogenic axis in breast cancer.  |  Mukhopadhyay, A., et al. 2013. Oncotarget. 4: 610-21. PMID: 23592399
12. CRISPR/Cas9-mediated knockout of the PDEF gene inhibits migration and invasion of human gastric cancer AGS cells.  |  Zhang, YQ., et al. 2019. Biomed Pharmacother. 111: 76-85. PMID: 30576937

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDEF Antibody (G-10) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-PDEF Antibody (G-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDEF Antibody (G-10) Specifications

Alternate Product Name: PDEF

Product Clone Name: G-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-250

Epitope Target: PDEF

Epitope Species: human

Product Reactivity/Detection: PDEF

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for PDEF Antibody (G-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent