PDE5A Antibody (D-3): sc-398747

PDE5A Antibody (D-3) is a mouse monoclonal IgG1 kappa light chain antibody that detects PDE5A protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDE5A Antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDE5A protein plays a crucial role in regulating intracellular levels of cyclic guanosine monophosphate (cGMP), which is vital for numerous physiological processes, including smooth muscle relaxation and vasodilation. By catalyzing cGMP hydrolysis to 5′GMP, PDE5A helps modulate signaling pathways that influence cardiovascular function and neuronal signaling. Expression in key tissues such as heart, placenta, aortic smooth muscle cells, skeletal muscle, and pancreas underscores PDE5A′s importance in maintaining homeostasis and responding to physiological stimuli. PDE5A′s ability to regulate cyclic nucleotide levels makes this protein a significant target for therapeutic interventions in conditions such as erectile dysfunction and pulmonary hypertension, highlighting anti-PDE5A antibody (D-3)′s relevance in both research and clinical applications.

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PDE5A Antibody (D-3) References:

1. Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase.  |  Hetman, JM., et al. 2000. Proc Natl Acad Sci U S A. 97: 472-6. PMID: 10618442
2. Reversal of neurobehavioral social deficits in dystrophic mice using inhibitors of phosphodiesterases PDE5A and PDE9A.  |  Alexander, MS., et al. 2016. Transl Psychiatry. 6: e901. PMID: 27676442
3. MicroRNA-19a/b-3p protect the heart from hypertension-induced pathological cardiac hypertrophy through PDE5A.  |  Liu, K., et al. 2018. J Hypertens. 36: 1847-1857. PMID: 29664809
4. Burn-Induced Cardiac Mitochondrial Dysfunction via Interruption of the PDE5A-cGMP-PKG Pathway.  |  Wen, JJ., et al. 2020. Int J Mol Sci. 21: PMID: 32231130
5. LncRNA HOXA-AS3 Promotes the Progression of Pulmonary Arterial Hypertension through Mediation of miR-675-3p/PDE5A Axis.  |  Li, ZK., et al. 2021. Biochem Genet. 59: 1158-1172. PMID: 33687636
6. Analysis of Geometric and Hemodynamic Profiles in Rat Arteriovenous Fistula Following PDE5A Inhibition.  |  Northrup, H., et al. 2021. Front Bioeng Biotechnol. 9: 779043. PMID: 34926425
7. Multiomic analysis of papillary thyroid cancers identifies BAIAP2L1-BRAF fusion and requirement of TRIM25, PDE5A and PKCδ for tumorigenesis.  |  Renaud, E., et al. 2022. Mol Cancer. 21: 195. PMID: 36217175
8. LncRNA ARAP1-AS1 targets miR-516b-5p/PDE5A axis to facilitate the progression of thyroid cancer.  |  Du, Q., et al. 2023. Anticancer Drugs. 34: 735-746. PMID: 36730555
9. IncRNA XIST Stimulates Papillary Thyroid Cancer Development through the miR-330-3p/PDE5A Axis.  |  Cai, T., et al. 2023. Crit Rev Eukaryot Gene Expr. 33: 13-26. PMID: 37017666
10. Phosphodiesterase profile of human B lymphocytes from normal and atopic donors and the effects of PDE inhibition on B cell proliferation.  |  Gantner, F., et al. 1998. Br J Pharmacol. 123: 1031-8. PMID: 9559883
11. Isolation and characterization of PDE8A, a novel human cAMP-specific phosphodiesterase.  |  Fisher, DA., et al. 1998. Biochem Biophys Res Commun. 246: 570-7. PMID: 9618252
12. Partial characterization of the active site human platelet cAMP phosphodiesterase, PDE3A, by site-directed mutagenesis.  |  Cheung, PP., et al. 1998. Arch Biochem Biophys. 360: 99-104. PMID: 9826434

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PDE5A Antibody (D-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-PDE5A Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PDE5A Antibody (D-3) Specifications

Alternate Product Name: PDE5A

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 31-150

Epitope Target: PDE5A

Epitope Species: human

Product Reactivity/Detection: PDE5A isoforms 1 and 2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for PDE5A Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin