PDCL3 Antibody (F-4): sc-515739
- PDCL3 Antibody (F-4) is a mouse monoclonal IgG2a κ PDCL3 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 181-198 within an internal region of PDCL3 of human origin
- PDCL3 Antibody (F-4) is recommended for detection of PDCL3 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-PDCL3 Antibody (F-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for PDCL3 Antibody (F-4) for WB applications. These reagents are now offered in bundles with PDCL3 Antibody (F-4) (see ordering information below).
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PDCL3 Antibody (F-4) is a mouse monoclonal IgG2a kappa light chain antibody that detects PDCL3 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PDCL3 (F-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PDCL3, also known as phosducin-like 3, VIAF1 (viral IAP-associated factor 1), VIAF, PHLP3, or HTPHLP, plays a crucial role in chaperone-assisted folding of proteins, particularly β Tubulin and Actin, which are essential for cell cycle regulation. PDCL3 is primarily located in the cytoplasm, where PDCL3 associates with the cytosolic chaperonin complex (CCT) to facilitate proper protein folding. PDCL3 has been shown to repress the ATPase activity of CCT, which can disrupt the folding of its substrates, thereby influencing cellular processes such as mitosis and cytoskeletal organization. PDCL3′s ability to modulate protein folding highlights its importance in maintaining cellular homeostasis and responding to stress, making PDCL3 a significant target for research in cell biology and related fields.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PDCL3 Antibody (F-4) References:
- Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins. | Blaauw, M., et al. 2003. EMBO J. 22: 5047-57. PMID: 14517243
- The C-terminal domain of viral IAP associated factor (cVIAF) is a structural homologue of phosducin: resonance assignments and secondary structure of the C-terminal domain of VIAF. | Jacob, J., et al. 2004. J Biomol NMR. 28: 197-8. PMID: 14755167
- VIAF, a conserved inhibitor of apoptosis (IAP)-interacting factor that modulates caspase activation. | Wilkinson, JC., et al. 2004. J Biol Chem. 279: 51091-9. PMID: 15371430
- PhLP3 modulates CCT-mediated actin and tubulin folding via ternary complexes with substrates. | Stirling, PC., et al. 2006. J Biol Chem. 281: 7012-21. PMID: 16415341
- Phosducin-like protein levels in leukocytes of patients with major depression and in rat cortex: the effect of chronic treatment with antidepressants. | Matuzany-Ruban, A., et al. 2006. Psychiatry Res. 141: 287-94. PMID: 16510194
- Function of phosducin-like proteins in G protein signaling and chaperone-assisted protein folding. | Willardson, BM. and Howlett, AC. 2007. Cell Signal. 19: 2417-27. PMID: 17658730
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDCL3 Antibody (F-4) | sc-515739 | 200 µg/ml | $322.00 | |||
PDCL3 Antibody (F-4): m-IgG Fc BP-HRP Bundle | sc-531492 | 200 µg Ab; 10 µg BP | $361.00 | |||
PDCL3 Antibody (F-4): m-IgGκ BP-HRP Bundle | sc-525191 | 200 µg Ab, 40 µg BP | $361.00 | |||
PDCL3 Antibody (F-4): m-IgG2a BP-HRP Bundle | sc-547802 | 200 µg Ab; 10 µg BP | $361.00 | |||
PDCL3 Antibody (F-4) AC | sc-515739 AC | 500 µg/ml, 25% agarose | $424.00 | |||
PDCL3 Antibody (F-4) HRP | sc-515739 HRP | 200 µg/ml | $322.00 | |||
PDCL3 Antibody (F-4) FITC | sc-515739 FITC | 200 µg/ml | $336.00 | |||
PDCL3 Antibody (F-4) PE | sc-515739 PE | 200 µg/ml | $349.00 | |||
PDCL3 Antibody (F-4) Alexa Fluor® 488 | sc-515739 AF488 | 200 µg/ml | $364.00 | |||
PDCL3 Antibody (F-4) Alexa Fluor® 546 | sc-515739 AF546 | 200 µg/ml | $364.00 | |||
PDCL3 Antibody (F-4) Alexa Fluor® 594 | sc-515739 AF594 | 200 µg/ml | $364.00 | |||
PDCL3 Antibody (F-4) Alexa Fluor® 647 | sc-515739 AF647 | 200 µg/ml | $364.00 | |||
PDCL3 Antibody (F-4) Alexa Fluor® 680 | sc-515739 AF680 | 200 µg/ml | $364.00 | |||
PDCL3 Antibody (F-4) Alexa Fluor® 790 | sc-515739 AF790 | 200 µg/ml | $364.00 |