PDC-E2 Antibody (A-9): sc-365276
- PDC-E2 Antibody (A-9) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 83-117 within an internal region of PDC-E2 of human origin
- recommended for detection of PDC-E2 of mouse, rat, human and avian origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and canine, porcine and avian
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PDC-E2 Antibody (A-9) for WB and IHC(P) applications. These reagents are now offered in bundles with PDC-E2 Antibody (A-9) (see ordering information below).
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PDC-E2 Antibody (A-9) is a mouse monoclonal IgG1 antibody that detects PDC-E2 in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). PDC-E2 plays a crucial role in cellular metabolism as a key component of the pyruvate dehydrogenase multienzyme complex, which is essential for converting pyruvate into acetyl-CoA, a vital substrate for the citric acid cycle. This conversion is not only critical for energy production but also for the synthesis of fatty acids and amino acids, making PDC-E2 integral to maintaining metabolic homeostasis. PDC-E2′s structure includes both an amino-terminal lipoyl-bearing domain and a carboxy-terminal catalytic domain, with the lipoyl domain being particularly important for enzymatic activity. The presence of autoantibodies against PDC-E2 in patients with primary biliary cirrhosis highlights PDC-E2′s significance in autoimmune responses, as these autoantibodies can lead to the destruction of biliary epithelial cells, further emphasizing PDC-E2′s importance in both health and disease. Anti-PDC-E2 antibody (A-9) is an invaluable tool for researchers studying metabolic disorders and autoimmune diseases, providing insights into the underlying mechanisms of these conditions.
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PDC-E2 Antibody (A-9) References:
- Quantitative and functional analysis of PDC-E2-specific autoreactive cytotoxic T lymphocytes in primary biliary cirrhosis. | Kita, H., et al. 2002. J Clin Invest. 109: 1231-40. PMID: 11994412
- Sidechain biology and the immunogenicity of PDC-E2, the major autoantigen of primary biliary cirrhosis. | Mao, TK., et al. 2004. Hepatology. 40: 1241-8. PMID: 15558739
- Catalytic domain of PDC-E2 contains epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis. | Braun, S., et al. 2010. World J Gastroenterol. 16: 973-81. PMID: 20180236
- Nuclear localization of pyruvate dehydrogenase complex-E2 (PDC-E2), a mitochondrial enzyme, and its role in signal transducer and activator of transcription 5 (STAT5)-dependent gene transcription. | Chueh, FY., et al. 2011. Cell Signal. 23: 1170-8. PMID: 21397011
- Antibodies to post-translationally modified mitochondrial peptide PDC-E2(167-184) in type 1 diabetes. | Nuti, F., et al. 2018. Arch Biochem Biophys. 659: 66-74. PMID: 30266625
- p-STAT3 is a PDC-E2 interacting partner in human cholangiocytes and hepatocytes with potential pathobiological implications. | Kilanczyk, E., et al. 2021. Sci Rep. 11: 21649. PMID: 34737337
- Identification of a novel PDC-E2 epitope in primary biliary cholangitis: Application for engineered Treg therapy. | Tewari, R., et al. 2024. J Autoimmun. 149: 103327. PMID: 39476446
- Abnormal expression of PDC-E2 on the apical surface of biliary epithelial cells in patients with antimitochondrial antibody-negative primary biliary cirrhosis. | Tsuneyama, K., et al. 1995. Hepatology. 22: 1440-6. PMID: 7590661
- Random phage mimotopes recognized by monoclonal antibodies against the pyruvate dehydrogenase complex-E2 (PDC-E2). | Cha, S., et al. 1996. Proc Natl Acad Sci U S A. 93: 10949-54. PMID: 8855289
- Inhibition of PDC-E2 human combinatorial autoantibodies by peptide mimotopes. | Leung, PS., et al. 1996. J Autoimmun. 9: 785-93. PMID: 9115581
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PDC-E2 Antibody (A-9) | sc-365276 | 200 µg/ml | $322.00 | |||
PDC-E2 Antibody (A-9): m-IgG Fc BP-HRP Bundle | sc-540325 | 200 µg Ab; 10 µg BP | $361.00 | |||
PDC-E2 Antibody (A-9): m-IgG1 BP-HRP Bundle | sc-542042 | 200 µg Ab; 20 µg BP | $361.00 | |||
PDC-E2 (A-9) Neutralizing Peptide | sc-365276 P | 100 µg/0.5 ml | $69.00 |