PCSK9 Antibody (F-8): sc-515082
- PCSK9 Antibody (F-8) is a mouse monoclonal IgG1 κ PCSK9 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against amino acids 175-334 mapping within an internal region of PCSK9 of human origin
- PCSK9 Antibody (F-8) is recommended for detection of PCSK9 of human origin by WB, IP, IF and ELISA
- Anti-PCSK9 Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PCSK9 (F-8): sc-515082.
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PCSK9 Antibody (F-8) for WB applications. These reagents are now offered in bundles with PCSK9 Antibody (F-8) (see ordering information below).
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SEE ALSO...
PCSK9 Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects PCSK9 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). PCSK9 (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC-1, is a 692 amino acid protein that belongs to the peptidase S8 family and features a single peptidase S8 domain. PCSK9 plays a crucial role in regulating plasma cholesterol levels by binding to low-density lipoprotein receptors, such as LDLR, very low-density lipoprotein receptor (VLDLR), and apolipoprotein receptor 2 (ApoER2), leading to their degradation in intracellular acidic compartments. PCSK9′s importance in cholesterol metabolism is underscored by involvement in the pathogenesis of familial hypercholesterolemia, a genetic disorder characterized by high cholesterol levels and increased risk of cardiovascular disease. PCSK9 is expressed in various tissues, including Schwann cells, neuro-epithelioma, colon carcinoma, and hepatic and pancreatic cell lines, with levels in the brain peaking in the cerebellum during perinatal development. Ischemic conditions can elevate PCSK9 levels in the adult brain, indicating a potential role in neuronal differentiation and apoptosis. Defects in the gene encoding PCSK9 are linked to autosomal dominant familial hypercholesterolemia 3 (HCHOLA3), highlighting PCSK9′s significance in both metabolic and neurological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PCSK9 Antibody (F-8) References:
- Mutations in PCSK9 cause autosomal dominant hypercholesterolemia. | Abifadel, M., et al. 2003. Nat Genet. 34: 154-6. PMID: 12730697
- Functional characterization of Narc 1, a novel proteinase related to proteinase K. | Naureckiene, S., et al. 2003. Arch Biochem Biophys. 420: 55-67. PMID: 14622975
- Decreased plasma cholesterol and hypersensitivity to statins in mice lacking Pcsk9. | Rashid, S., et al. 2005. Proc Natl Acad Sci U S A. 102: 5374-9. PMID: 15805190
- Sequence variations in PCSK9, low LDL, and protection against coronary heart disease. | Cohen, JC., et al. 2006. N Engl J Med. 354: 1264-72. PMID: 16554528
- Implication of the proprotein convertase NARC-1/PCSK9 in the development of the nervous system. | Poirier, S., et al. 2006. J Neurochem. 98: 838-50. PMID: 16893422
- Molecular biology of PCSK9: its role in LDL metabolism. | Horton, JD., et al. 2007. Trends Biochem Sci. 32: 71-7. PMID: 17215125
- PCSK9 regulates neuronal apoptosis by adjusting ApoER2 levels and signaling. | Kysenius, K., et al. 2012. Cell Mol Life Sci. 69: 1903-16. PMID: 22481440
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCSK9 Antibody (F-8) | sc-515082 | 200 µg/ml | $316.00 | |||
PCSK9 Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-527979 | 200 µg Ab; 10 µg BP | $354.00 | |||
PCSK9 Antibody (F-8): m-IgG1 BP-HRP Bundle | sc-533352 | 200 µg Ab; 20 µg BP | $354.00 | |||
PCSK9 Antibody (F-8) AC | sc-515082 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PCSK9 Antibody (F-8) HRP | sc-515082 HRP | 200 µg/ml | $316.00 | |||
PCSK9 Antibody (F-8) FITC | sc-515082 FITC | 200 µg/ml | $330.00 | |||
PCSK9 Antibody (F-8) PE | sc-515082 PE | 200 µg/ml | $343.00 | |||
PCSK9 Antibody (F-8) Alexa Fluor® 488 | sc-515082 AF488 | 200 µg/ml | $357.00 | |||
PCSK9 Antibody (F-8) Alexa Fluor® 546 | sc-515082 AF546 | 200 µg/ml | $357.00 | |||
PCSK9 Antibody (F-8) Alexa Fluor® 594 | sc-515082 AF594 | 200 µg/ml | $357.00 | |||
PCSK9 Antibody (F-8) Alexa Fluor® 647 | sc-515082 AF647 | 200 µg/ml | $357.00 | |||
PCSK9 Antibody (F-8) Alexa Fluor® 680 | sc-515082 AF680 | 200 µg/ml | $357.00 | |||
PCSK9 Antibody (F-8) Alexa Fluor® 790 | sc-515082 AF790 | 200 µg/ml | $357.00 |