PCNA Antibody (F-2): sc-25280
- Proliferating cell nuclear antigen (F-2) is a mouse monoclonal IgG2a κ, cited in 391 publications, provided at 200 µg/ml
- Recommended for detection in mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti Proliferating cell nuclear antigen Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of sc-25280.
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SEE ALSO...
Proliferating Cell Nuclear Antigen, also known as cyclin, is a highly conserved nuclear protein that plays a critical role in DNA replication and repair. Functioning as a sliding clamp, Proliferating Cell Nuclear Antigen forms a homotrimeric ring around DNA, facilitating the processivity of DNA polymerases delta (δ) and epsilon (ε) during replication. This allows Proliferating Cell Nuclear Antigen to act as a scaffold for recruiting various proteins that are key players in DNA repair, chromatin remodeling, and the regulation of the cell cycle. Proliferating Cell Nuclear Antigen expression peaks during the S phase of the cell cycle, making it a useful marker for cell proliferation studies. Proliferating Cell Nuclear Antigen is widely used in research to investigate the mechanisms governing DNA replication, genome stability, and cellular responses to DNA damage. Playing a crucial role in various DNA repair pathways, such as nucleotide excision repair, base excision repair, and mismatch repair, Proliferating Cell Nuclear Antigen interacts with key proteins to fix DNA lesions and correct base-pair mismatches after replication. Proliferating Cell Nuclear Antigen is involved in chromatin assembly and nucleosome formation, which are critical for maintaining genomic integrity during replication and repair processes. Post-translational modifications, such as ubiquitination and sumoylation, play a significant role in regulating Proliferating Cell Nuclear Antigen’s functions, particularly in DNA damage tolerance pathways like translesion synthesis and template switching. These modifications ensure that Proliferating Cell Nuclear Antigen can function effectively even when the cell encounters damaged DNA, aiding in genome maintenance. In cancer research, Proliferating Cell Nuclear Antigen is particularly important due to its overexpression in highly proliferative tumor cells. The high proliferation rates of cancer cells make Proliferating Cell Nuclear Antigen a significant biomarker for both diagnosis and prognosis. Proliferating Cell Nuclear Antigen also serves as a promising target for developing novel cancer therapies, as modulating Proliferating Cell Nuclear Antigen or its interactions could inhibit tumor growth.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PCNA Antibody (F-2) | sc-25280 | 200 µg/ml | $316.00 | |||
PCNA Antibody (F-2): m-IgG Fc BP-HRP Bundle | sc-528392 | 200 µg Ab; 10 µg BP | $354.00 | |||
PCNA Antibody (F-2): m-IgGκ BP-HRP Bundle | sc-520762 | 200 µg Ab, 40 µg BP | $354.00 | |||
PCNA Antibody (F-2): m-IgG2a BP-HRP Bundle | sc-547083 | 200 µg Ab; 10 µg BP | $354.00 | |||
PCNA Antibody (F-2) AC | sc-25280 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PCNA Antibody (F-2) HRP | sc-25280 HRP | 200 µg/ml | $316.00 | |||
PCNA Antibody (F-2) FITC | sc-25280 FITC | 200 µg/ml | $330.00 | |||
PCNA Antibody (F-2) PE | sc-25280 PE | 200 µg/ml | $343.00 | |||
PCNA Antibody (F-2) Alexa Fluor® 488 | sc-25280 AF488 | 200 µg/ml | $357.00 | |||
PCNA Antibody (F-2) Alexa Fluor® 546 | sc-25280 AF546 | 200 µg/ml | $357.00 | |||
PCNA Antibody (F-2) Alexa Fluor® 594 | sc-25280 AF594 | 200 µg/ml | $357.00 | |||
PCNA Antibody (F-2) Alexa Fluor® 647 | sc-25280 AF647 | 200 µg/ml | $357.00 | |||
PCNA Antibody (F-2) Alexa Fluor® 680 | sc-25280 AF680 | 200 µg/ml | $357.00 | |||
PCNA Antibody (F-2) Alexa Fluor® 790 | sc-25280 AF790 | 200 µg/ml | $357.00 |