PCNA Antibody (F-2) recommended for detection of PCNA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PCNA Antibody (F-2): sc-25280

PCNA Antibody (F-2) is rated 5.0 out of 5 by 21.
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Datasheets

    • PCNA Antibody (F-2) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • Anti-PCNA Antibody (F-2) is recommended for detection of PCNA of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-PCNA Antibody (F-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PCNA (F-2): sc-25280.

请问如何获取试用装?

Asked by: yousmile
感谢您的提问。目前我们可以提供该抗体的10 µg 试用装,详情请咨询我们的技术服务部门。亚洲技术服务电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”。Thank you for your question. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department to request a sample. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 8
Date published: 2018-12-21

Hello, Im interested in this PCNA antibody but I don't want to purchase a full size vial until im sure it will work on my sample. Do you sell small volume samples? Thanks

Asked by: Aliciastl
Thank you for your question. For a limited time, we are offering a free 10 µg sample of this mouse monoclonal antibody, PCNA (F-2): sc-25280. To request a free sample to test before you purchase the antibody, please contact our Technical Service Department. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-03-08

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-30
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Rated 5 out of 5 by from 免疫染色に優れています HCT116細胞を4%PFAで固定し、この抗体を200倍希釈で使用しました。免疫染色ではきれいな結果が得られました。PCNA(PC10)に関しも、同様に良い結果が得られました。
Date published: 2020-03-28
Rated 5 out of 5 by from Great product I bought this antibody several weeks ago. It works good on rat tissue.
Date published: 2019-04-22
Rated 5 out of 5 by from Works great with both explant and cell cultures I used PCNA antibody in the SCN explant cultures and OPC cultures. Works really great with a strong signal in both types of experiments.
Date published: 2018-06-05
Rated 5 out of 5 by from Strong signal This antibody gives very stron signal even at the dilution as high as 1:5000.
Date published: 2018-03-12
Rated 4 out of 5 by from Worked on regenerating liver tissue lysates I was able to see increased expression of PCNA in regenerating liver lysates as compared to controls. This had been difficult to see with other antibodies whose background bands cloud the protein of interest.
Date published: 2017-08-25
Rated 5 out of 5 by from good reference control PCNA antibody is a good reference control for nuclear protein, we used this antibody diluted 1:1000, we see the specific bands at 36kda,recommend diluted the antibody not more than 1:2000.
Date published: 2017-08-12
Rated 5 out of 5 by from western blot western blot analysis and works well at 1:800 dilution in human cell,Great antibody.
Date published: 2017-05-13
Rated 5 out of 5 by from strong band at 34kda. Good quality for western blotting with human breast cancer tissue detection,shows powerful band.
Date published: 2017-05-13
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