PCMT1 Antibody (D-E7) recommended for detection of PCMT1 of mouse, rat and human origin by WB and IP

PCMT1 Antibody (D-E7): sc-100977

PCMT1 Antibody (D-E7) is rated 4.8 out of 5 by 4.
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Datasheets

    • Anti-PCMT1 Antibody (D-E7) is a mouse monoclonal IgG1 κ PCMT1 antibody, cited in 4 publications, provided at 100 µg/ml
    • raised against recombinant PCMT1 of human origin
    • recommended for detection of PCMT1 of mouse, rat and human origin by WB and IP
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PCMT1 (D-E7): sc-100977.

I am using PCMT1 (D-E7): sc-100977 antibody for immunoprecipitation, how much antibody should I use?

Asked by: Dr Ninau Qelp
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from wonderful antibody 1:500 diluted for wb of rat protein,the band is special and strong
Date published: 2018-03-19
Rated 4 out of 5 by from Buen resultado Muy contenta con este anticuerpo. Lo he probado para WB (50ug muestra, concentración 1:500, 1% BSA en PBS), con muestras de ratón y funciona perfectamente.
Date published: 2017-08-01
Rated 5 out of 5 by from Reliable Antibody, Great for WB Great antibody for WB. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-18
Rated 5 out of 5 by from WB publishable data with human BJ: foreskin WB publishable data with human BJ: foreskin fibroblast; U2OS, SH-SY5Y, NTERA2, HCT116, HeLa, and NCCIT - SCBT Publication Review
Date published: 2013-06-30
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