Date published: 2026-6-4

1-800-457-3801

SCBT Portrait Logo
Seach Input

PCB Antibody (D-9): sc-365673

5.0(5)
Write a reviewAsk a question

Datasheets
  • PCB Antibody (D-9) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
  • raised against amino acids 879-1178 mapping at the C-terminus of PCB of human origin
  • Anti-PCB Antibody (D-9) is recommended for detection of PCB of mouse, rat and human origin by WB, IP, IF and ELISA
  • Anti-PCB Antibody (D-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for PCB Antibody (D-9) for WB applications. This reagent is now offered in a bundle with PCB Antibody (D-9) (see ordering information below).
Gene Editing Promo Banner

QUICK LINKS

SEE ALSO...

PCB Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects pyruvate carboxylase (PCB) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PCB antibody (D-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Pyruvate carboxylase plays a crucial role in cellular metabolism by catalyzing the conversion of pyruvate to oxaloacetate, a key intermediate in the citric acid cycle, which is essential for energy production and biosynthesis. PCB (D-9) antibody targets an enzyme that′s particularly important in gluconeogenesis and fatty acid synthesis, making PCB vital for maintaining metabolic homeostasis. PCB monoclonal antibody (D-9) recognizes a mitochondrial protein involved in regulating the balance between energy production and consumption, highlighting PCB′s significance in metabolic disorders. Mutations affecting PCB function can lead to severe metabolic conditions, including lactic acidosis and hypoglycemia, underscoring the importance of studying PCB in various biological contexts.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

PCB Antibody (D-9)

sc-365673
200 µg/ml
$322.00

PCB Antibody (D-9): m-IgG Fc BP-HRP Bundle

sc-525895
200 µg Ab; 10 µg BP
$361.00

PCB Antibody (D-9) AC

sc-365673 AC
500 µg/ml, 25% agarose
$424.00

PCB Antibody (D-9) HRP

sc-365673 HRP
200 µg/ml
$322.00

PCB Antibody (D-9) FITC

sc-365673 FITC
200 µg/ml
$336.00

PCB Antibody (D-9) PE

sc-365673 PE
200 µg/ml
$349.00

PCB Antibody (D-9) Alexa Fluor® 488

sc-365673 AF488
200 µg/ml
$364.00

PCB Antibody (D-9) Alexa Fluor® 546

sc-365673 AF546
200 µg/ml
$364.00

PCB Antibody (D-9) Alexa Fluor® 594

sc-365673 AF594
200 µg/ml
$364.00

PCB Antibody (D-9) Alexa Fluor® 647

sc-365673 AF647
200 µg/ml
$364.00

PCB Antibody (D-9) Alexa Fluor® 680

sc-365673 AF680
200 µg/ml
$364.00

PCB Antibody (D-9) Alexa Fluor® 790

sc-365673 AF790
200 µg/ml
$364.00

What is the recommended fixation method for immunofluorescence staining with PCB (D-9): sc-365673 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2026, m_6, d_3, h_11CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.46
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_365673, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 110ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Excellent antibody for WB20ug of MDA-MB231 whole cell lysate probed at 1/200X dilution produce excellent results. PC signal was strong and clear.
Date published: 2020-08-14
Rated 5 out of 5 by from works well1/1000 dilution in 3% BSA works well for western blotting
Date published: 2018-03-01
Rated 5 out of 5 by from Performed well in WB in human liver tissuePerformed well in WB in human liver tissue extract and Hep G2 and HEK293 whole cell lysates. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Strong band of interest observed in WB inStrong band of interest observed in WB in mouse PCB transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-02-16
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic stainingGreat immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-22
  • y_2026, m_6, d_3, h_11
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.46
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_en_US, sid_365673, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 9ms
  • REVIEWS, PRODUCT
PCB Antibody (D-9) is rated 5.0 out of 5 by 5.
  • y_2026, m_6, d_3, h_11
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.46
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_en_US, sid_365673, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 152ms
  • REVIEWS, PRODUCT