PBEF Antibody (H-11): sc-166946
- PBEF Antibody (H-11) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 427-446 near the C-terminus of PBEF of human origin
- recommended for detection of Pre-B cell enhancing factor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, canine and bovine
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for PBEF Antibody (H-11) for WB and IHC(P) applications. These reagents are now offered in bundles with PBEF Antibody (H-11) (see ordering information below).
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PBEF Antibody (H-11) is a mouse monoclonal IgG1 antibody that detects pre-B cell-enhancing factor (PBEF) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PBEF, also known as nicotinamide phosphoribosyltransferase (Nampt) or visfatin, plays a crucial role in NAD biosynthesis, catalyzing the conversion of nicotinamide and 5-phosphoribosyl-1-pyrophosphate into nicotinamide mononucleotide, a key precursor in NAD production. NAD is essential for various cellular functions, including energy metabolism and DNA repair. PBEF is highly enriched in visceral fat, and plasma expression levels increase during obesity, linking PBEF to metabolic disorders. PBEF primarily localizes in the cytoplasm and shows predominant expression in bone marrow, muscle, and liver tissues, with additional presence in placenta, lung, kidney, and heart, highlighting diverse biological significance across different tissues. Anti-PBEF antibody (H-11) serves as an invaluable tool for researchers studying PBEF′s role in metabolic regulation and obesity-related conditions.
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PBEF Antibody (H-11) References:
- Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme. | Revollo, JR., et al. 2007. Cell Metab. 6: 363-75. PMID: 17983582
- Plasma levels of PBEF/Nampt/visfatin are decreased in patients with liver cirrhosis. | de Boer, JF., et al. 2009. Am J Physiol Gastrointest Liver Physiol. 296: G196-201. PMID: 19074645
- Visfatin/PBEF and atherosclerosis-related diseases. | Filippatos, TD., et al. 2010. Curr Vasc Pharmacol. 8: 12-28. PMID: 19485930
- Maternal plasma concentration of the pro-inflammatory adipokine pre-B-cell-enhancing factor (PBEF)/visfatin is elevated in pregnant patients with acute pyelonephritis. | Mazaki-Tovi, S., et al. 2010. Am J Reprod Immunol. 63: 252-62. PMID: 20085562
- Leucocytes are a major source of circulating nicotinamide phosphoribosyltransferase (NAMPT)/pre-B cell colony (PBEF)/visfatin linking obesity and inflammation in humans. | Friebe, D., et al. 2011. Diabetologia. 54: 1200-11. PMID: 21298414
- Nampt/PBEF/visfatin upregulation in colorectal tumors, mirrored in normal tissue and whole blood of colorectal cancer patients, is associated with metastasis, hypoxia, IL1β, and anemia. | Neubauer, K., et al. 2015. Biomed Res Int. 2015: 523930. PMID: 26075243
- Unique Toll-Like Receptor 4 Activation by NAMPT/PBEF Induces NFκB Signaling and Inflammatory Lung Injury. | Camp, SM., et al. 2015. Sci Rep. 5: 13135. PMID: 26272519
- Relationship between NAMPT/PBEF/visfatin and prognosis of patients with malignant tumors: a systematic review and meta-analysis. | Ji, C., et al. 2019. Ann Transl Med. 7: 785. PMID: 32042801
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PBEF Antibody (H-11) | sc-166946 | 200 µg/ml | $322.00 | |||
PBEF Antibody (H-11): m-IgG Fc BP-HRP Bundle | sc-540178 | 200 µg Ab; 10 µg BP | $361.00 | |||
PBEF Antibody (H-11): m-IgG1 BP-HRP Bundle | sc-541952 | 200 µg Ab; 20 µg BP | $361.00 | |||
PBEF (H-11) Neutralizing Peptide | sc-166946 P | 100 µg/0.5 ml | $69.00 |