PBEF Antibody (1D3A12): sc-130058

PBEF Antibody (1D3A12) is a mouse monoclonal IgG1 antibody that detects pre-B cell-enhancing factor (PBEF) in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). PBEF, also known as nicotinamide phosphoribosyltransferase (Nampt) or visfatin, plays a crucial role in NAD biosynthesis, catalyzing the conversion of nicotinamide and 5-phosphoribosyl-1-pyrophosphate into nicotinamide mononucleotide, a key intermediate in the NAD synthesis pathway. This enzymatic activity represents a rate-limiting step in NAD production, essential for various cellular processes, including energy metabolism and DNA repair. PBEF (1D3A12) antibody detects high expression in visceral fat, with plasma levels increasing during obesity, linking PBEF to metabolic regulation and potential implications in obesity-related diseases. PBEF localizes primarily in the cytoplasm and shows predominant expression in bone marrow, muscle, and liver tissues, with additional detection in placenta, lung, kidney, and heart, highlighting diverse biological roles across different tissues.

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PBEF Antibody (1D3A12) References:

1. Pre-B-cell colony-enhancing factor (PBEF/Visfatin) gene expression is modulated by NF-kappaB and AP-1 in human amniotic epithelial cells.  |  Kendal, CE. and Bryant-Greenwood, GD. 2007. Placenta. 28: 305-14. PMID: 16701870
2. Pre-B cell colony-enhancing factor (PBEF)/visfatin: a novel mediator of innate immunity.  |  Luk, T., et al. 2008. J Leukoc Biol. 83: 804-16. PMID: 18252866
3. Stretch and inflammation-induced Pre-B cell colony-enhancing factor (PBEF/Visfatin) and Interleukin-8 in amniotic epithelial cells.  |  Kendal-Wright, CE., et al. 2010. Placenta. 31: 665-74. PMID: 20598369
4. Pre-B cell colony-enhancing factor (PBEF/Nampt/visfatin) primes neutrophils for augmented respiratory burst activity through partial assembly of the NADPH oxidase.  |  Malam, Z., et al. 2011. J Immunol. 186: 6474-84. PMID: 21518975
5. Pleiotropic functions of pre-B-cell colony-enhancing factor (PBEF) revealed by transcriptomics of human pulmonary microvascular endothelial cells treated with PBEFsiRNA.  |  Cheranova, D., et al. 2012. Genes Cells. 17: 420-30. PMID: 22487217
6. Visfatin/pre-B-cell colony-enhancing factor (PBEF), a proinflammatory and cell motility-changing factor in rheumatoid arthritis.  |  Meier, FM., et al. 2012. J Biol Chem. 287: 28378-85. PMID: 22767598
7. Pre-B cell colony enhancing factor (PBEF/NAMPT/Visfatin) and vascular endothelial growth factor (VEGF) cooperate to increase the permeability of the human placental amnion.  |  Astern, JM., et al. 2013. Placenta. 34: 42-9. PMID: 23151382
8. Pre-B cell colony enhancing factor (PBEF), a cytokine with multiple physiological functions.  |  Sun, Z., et al. 2013. Cytokine Growth Factor Rev. 24: 433-42. PMID: 23787158
9. Pre-B-cell colony enhancing factor (PBEF) increases endothelial permeability in hypoxia/re-oxygenation model.  |  Yang, W., et al. 2015. Int J Clin Exp Med. 8: 8842-7. PMID: 26309537
10. The role of visfatin (PBEF/Nampt) in pregnancy complications.  |  Pavlová, T., et al. 2015. J Reprod Immunol. 112: 102-10. PMID: 26451650

The preferred PBEF antibody is PBEF Antibody (E-3): sc-393444.