paxillin Antibody (D-9): sc-365174

paxillin Antibody (D-9) is a mouse monoclonal IgG1 kappa light chain antibody raised against amino acids 155-268, mapping within an internal region of human paxillin. Paxillin, also known as PXN, is a focal adhesion phosphoprotein localized to the cytoskeleton, playing a crucial role in cell movement, adhesion, and signal transduction pathways. Structurally, paxillin contains five N-terminal LD motifs and four C-terminal LIM domains, zinc-binding structures that facilitate protein-protein interactions. This modular architecture allows paxillin to function as a scaffold protein, assembling various signaling molecules at focal adhesion sites. The phosphorylation of paxillin on tyrosine and serine residues by kinases such as FAK (focal adhesion kinase) and PYK2 modulates its interactions and activity, influencing processes like cell migration and morphology. Phosphorylation at specific sites, such as Tyr118, occurs in response to stimuli like PDGF treatment and v-src transformation, although FAK phosphorylation is not required for paxillin′s recruitment to adhesion sites. During mitosis, paxillin levels decrease due to proteolytic downregulation, and paxillin undergoes alternative phosphorylation on serine residues, highlighting its role in cell cycle regulation. Anti-paxillin antibody (D-9) detects the α, β, and γ isoforms of paxillin from mouse, rat, and human origins in applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, paxillin (D-9) antibody is a valuable tool for researchers studying paxillin′s structure-function relationships and its role in cellular signaling pathways across different species.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-paxillin Antibody (D-9) has 17 citations in a variety of scientific publications.

Primary antibodies like Anti-paxillin Antibody (D-9) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

paxillin Antibody (D-9) Specifications

Alternate Product Name: paxillin

Product Clone Name: D-9

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 155-268

Epitope Target: paxillin

Epitope Species: human

Product Reactivity/Detection: α,β, γ isoforms of paxillin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for paxillin Antibody (D-9): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred paxillin antibody is paxillin Antibody (B-2): sc-365379.