paxillin Antibody (A-5): sc-390738

paxillin Antibody (A-5) is a mouse monoclonal IgG2b kappa light chain antibody that specifically targets an epitope mapping between amino acids 59-72 of human paxillin. Paxillin, also known as PXN, is a focal adhesion phosphoprotein localized to the cytoskeleton, playing a crucial role in cell adhesion, migration, and signal transduction pathways. Structurally, paxillin contains five LD motifs in its N-terminal region and four LIM domains in its C-terminal region. These domains facilitate interactions with various proteins such as focal adhesion kinase (FAK), Src family kinases, and integrins. The LD motifs serve as docking sites for proteins involved in cytoskeletal dynamics, while the LIM domains are zinc-binding structures that mediate protein-protein interactions, ensuring proper assembly of focal adhesion complexes. Phosphorylation of paxillin on specific tyrosine residues, notably Tyr118, by kinases like FAK and PYK2 modulates activity and interactions, influencing processes like cell morphology and motility. During mitosis, paxillin levels decrease due to proteolytic downregulation, and paxillin undergoes alternative phosphorylation on serine residues, highlighting a role in cell cycle regulation. anti-paxillin antibody (A-5) reacts with the α, β, and γ isoforms of paxillin from mouse, rat, and human origins in applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Understanding paxillin′s structure is important because alterations in domains can affect function in signal transduction pathways, potentially contributing to diseases such as cancer. paxillin (A-5) antibody is therefore a valuable tool for researchers studying the structural-functional relationships of paxillin in cellular processes.

The preferred paxillin antibody is paxillin Antibody (B-2): sc-365379.