Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Pax-9 CRISPR/Cas9 KO Plasmid (h): sc-403899
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pax-9 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pax-9 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pax-9 Antibody (7C2): sc-56823
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pax-9 CRISPR/Cas9 KO Plasmid (h) | sc-403899 | 20 µg | $397.00 |
Overview
PAX9 encodes the paired box transcription factor Pax-9, a nuclear DNA-binding regulator essential for embryonic patterning and organogenesis. Pax-9 coordinates transcriptional programs that govern craniofacial and pharyngeal development, odontogenesis, and epithelial–mesenchymal interactions, acting within broader developmental gene networks that include PAX family factors and homeobox pathways. In human genetics, altered PAX9 function is strongly associated with congenital tooth agenesis and related craniofacial anomalies, making it a key locus for studying developmental transcriptional control. In cellular models, Pax-9 provides a tractable node for dissecting lineage specification, morphogen-responsive gene regulation, and downstream differentiation cascades.
Pax-9 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PAX9 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PAX9 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PAX9 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pax-9 protein expression.
This CRISPR knockout system enables efficient generation of PAX9-deficient cell models for investigation of Pax-9 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PAX9 exon(s) critical for Pax-9 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PAX9 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pax-9 CRISPR/Cas9 KO Plasmid (h) and Pax-9 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PAX9 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pax-9 HDR Plasmid (h) and Pax-9 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PAX9 homology arms to support homology-directed repair at defined PAX9 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.