Pax-6 Antibody (AD2.35): sc-53108

Pax-6 Antibody (AD2.35) is a mouse monoclonal IgG2a kappa light chain antibody that detects Pax-6 in mouse, rat, human, and avian species through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Pax-6 antibody (AD2.35) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pax-6 plays a crucial role in eye development, functioning as a transcriptional regulator that binds to specific DNA sequences to control the expression of genes essential for the formation of ocular structures. Mutations in Pax-6 are linked to several congenital eye disorders, including aniridia, which is characterized by the absence of an iris and can lead to severe visual impairment. Pax-6′s paired domain structure consists of two distinct DNA-binding subdomains that allow for precise interaction with target genes, thereby influencing the developmental pathways that lead to proper eye formation. Understanding Pax-6 function and regulation is vital for elucidating the genetic basis of eye diseases and developing potential therapeutic strategies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Pax-6 Antibody (AD2.35) References:

1. Functional PAX-6 gene-linked polymorphic region: potential association with paranoid schizophrenia.  |  Stöber, G., et al. 1999. Biol Psychiatry. 45: 1585-91. PMID: 10376119
2. The pancreatic beta-cell-specific transcription factor Pax-4 inhibits glucagon gene expression through Pax-6.  |  Ritz-Laser, B., et al. 2002. Diabetologia. 45: 97-107. PMID: 11845228
3. DNA-binding characteristics of cnidarian Pax-C and Pax-B proteins in vivo and in vitro: no simple relationship with the Pax-6 and Pax-2/5/8 classes.  |  Plaza, S., et al. 2003. J Exp Zool B Mol Dev Evol. 299: 26-35. PMID: 14508814
4. Differential regulation of islet-specific glucose-6-phosphatase catalytic subunit-related protein gene transcription by Pax-6 and Pdx-1.  |  Martin, CC., et al. 2004. J Biol Chem. 279: 34277-89. PMID: 15180990
5. Pax-6 expression in posthatch chick retina during and recovery from form-deprivation myopia.  |  Bhat, SP., et al. 2004. Dev Neurosci. 26: 328-35. PMID: 15855761
6. Protein phosphatase-1 modulates the function of Pax-6, a transcription factor controlling brain and eye development.  |  Yan, Q., et al. 2007. J Biol Chem. 282: 13954-65. PMID: 17374606
7. Association analysis of a PAX-6 gene promoter-associated polymorphic repeat with alcohol dependence.  |  Samochowiec, J., et al. 1999. Addict Biol. 4: 323-8. PMID: 20575799
8. Sumoylation activates the transcriptional activity of Pax-6, an important transcription factor for eye and brain development.  |  Yan, Q., et al. 2010. Proc Natl Acad Sci U S A. 107: 21034-9. PMID: 21084637
9. Differential expression between PAX-6 and EN proteins in medulloblastoma.  |  Vincent, S., et al. 1996. Int J Oncol. 8: 901-10. PMID: 21544444
10. Pax-6 is essential for lens-specific expression of zeta-crystallin.  |  Richardson, J., et al. 1995. Proc Natl Acad Sci U S A. 92: 4676-80. PMID: 7753863

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pax-6 Antibody (AD2.35) has 24 citations in a variety of scientific publications.

Primary antibodies like Anti-Pax-6 Antibody (AD2.35) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pax-6 Antibody (AD2.35) Specifications

Alternate Product Name: Pax-6

Product Clone Name: AD2.35

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-206

Epitope Target: Pax-6

Epitope Species: human

Product Reactivity/Detection: Pax-6

Species Reactivity/Detection: mouse, rat, human, avian

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 24 citations in various scientific publications

Available Conjugates/Forms for Pax-6 Antibody (AD2.35): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Pax-6 antibody is Pax-6 Antibody (PAX6): sc-81649.