Pax-4 Antibody (3C12): sc-517254

Pax-4 Antibody (3C12) is a mouse monoclonal IgG1 antibody that detects Pax-4 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Pax-4 (3C12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pax-4 protein, also known as paired box gene 4, plays a crucial role in the differentiation of insulin-producing beta cells and is essential for normal pancreatic islet development. Located primarily in pancreatic islets, Pax-4 functions as a transcriptional repressor, binding to a common cis element in promoters of glucagon, insulin, and somatostatin, thereby regulating their expression. Pax-4 presence in pancreatic islets and specific β cell lines, such as MIN6, β TC, and NIT1, highlights its importance in endocrine pancreas differentiation, where Pax-4 may compete with Pax-6 for binding sites, thus influencing the transcriptional landscape of these critical hormones. Human Pax-4 gene encodes a deduced protein of 350 amino acids, exhibiting 80% identity with its mouse counterpart, underscoring the evolutionary conservation of its function in glucose homeostasis and metabolic regulation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Pax-4 Antibody (3C12) References:

1. Pax 4 and 6 regulate gastrointestinal endocrine cell development.  |  Larsson, LI., et al. 1998. Mech Dev. 79: 153-9. PMID: 10349628
2. DNA binding and transactivating properties of the paired and homeobox protein Pax4.  |  Kalousová, A., et al. 1999. Biochem Biophys Res Commun. 259: 510-8. PMID: 10364449
3. The pancreatic beta-cell-specific transcription factor Pax-4 inhibits glucagon gene expression through Pax-6.  |  Ritz-Laser, B., et al. 2002. Diabetologia. 45: 97-107. PMID: 11845228
4. Regulation of Pax4 paired homeodomain gene by neuron-restrictive silencer factor.  |  Kemp, DM., et al. 2003. J Biol Chem. 278: 35057-62. PMID: 12829700
5. The gene Pax4 is an essential regulator of pancreatic beta-cell development.  |  Sosa-Pineda, B. 2004. Mol Cells. 18: 289-94. PMID: 15650323
6. A focus on the role of Pax4 in mature pancreatic islet beta-cell expansion and survival in health and disease.  |  Brun, T. and Gauthier, BR. 2008. J Mol Endocrinol. 40: 37-45. PMID: 18234907
7. Pax4 acts as a key player in pancreas development and plasticity.  |  Napolitano, T., et al. 2015. Semin Cell Dev Biol. 44: 107-14. PMID: 26319183
8. PAX4 preserves endoplasmic reticulum integrity preventing beta cell degeneration in a mouse model of type 1 diabetes mellitus.  |  Mellado-Gil, JM., et al. 2016. Diabetologia. 59: 755-65. PMID: 26813254
9. PAX4 R192H and P321H polymorphisms in type 2 diabetes and their functional defects.  |  Sujjitjoon, J., et al. 2016. J Hum Genet. 61: 943-949. PMID: 27334367
10. Transcription factor PAX4 facilitates gastric cancer progression through interacting with miR-27b-3p/Grb2 axis.  |  Zhang, Y., et al. 2021. Aging (Albany NY). 13: 16786-16803. PMID: 34162761
11. PAX4 loss of function increases diabetes risk by altering human pancreatic endocrine cell development.  |  Lau, HH., et al. 2023. Nat Commun. 14: 6119. PMID: 37777536
12. Molecular cloning of mouse paired-box-containing gene (Pax)-4 from an islet beta cell line and deduced sequence of human Pax-4.  |  Matsushita, T., et al. 1998. Biochem Biophys Res Commun. 242: 176-80. PMID: 9439631

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pax-4 Antibody (3C12) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-Pax-4 Antibody (3C12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pax-4 Antibody (3C12) Specifications

Alternate Product Name: PAX4

Product Clone Name: 3C12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids: 105-232

Epitope Target: Pax-4

Epitope Species: human

Product Reactivity/Detection: Pax-4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Pax-4 Antibody (3C12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Pax antibody is Pax Antibody (D-7): sc-514352.