Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Pax-2 CRISPR/Cas9 KO Plasmid (m): sc-422115
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Pax-2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Pax-2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Pax-2 Antibody (60-P): sc-130387
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Pax-2 CRISPR/Cas9 KO Plasmid (m) | sc-422115 | 20 µg | $397.00 |
Overview
Mouse Pax2 encodes Pax-2, a paired box transcription factor that regulates gene expression programs during organogenesis, particularly in the developing kidney, optic nerve/retina, and inner ear. Pax-2 functions in lineage specification and epithelial differentiation by coordinating developmental transcriptional networks and crosstalk with pathways such as WNT and Notch signaling. Altered PAX2 activity is associated with congenital anomalies of the kidney and urinary tract and ocular developmental defects, making Pax2 a widely used marker and mechanistic node in developmental and disease-modeling studies. In cell systems, Pax-2 influences proliferation, morphogenesis, and maintenance of tissue-specific identity through direct DNA binding and transcriptional repression/activation complexes.
Pax-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Pax2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Pax2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Pax2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Pax-2 protein expression.
This CRISPR knockout system enables efficient generation of Pax2-deficient cell models for investigation of Pax-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Pax2 exon(s) critical for Pax-2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Pax2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Pax-2 CRISPR/Cas9 KO Plasmid (m) and Pax-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Pax2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Pax-2 HDR Plasmid (m) and Pax-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Pax2 homology arms to support homology-directed repair at defined Pax2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.