Pax-2/5/8 Antibody (G-3): sc-377181

Pax-2/5/8 Antibody (G-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects Pax-2/5/8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-Pax-2/5/8 antibody (G-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Pax-2/5/8 proteins play a crucial role in early developmental processes, particularly in the formation of the kidney, ureter, and various sensory organs. These proteins are transcription factors that contain paired domains, which are essential for their function in regulating gene expression during embryogenesis. PAX2 is expressed in primitive cells of the kidney, ureter, eye, ear, and central nervous system, highlighting PAX2′s importance in organogenesis. In human embryonic development, PAX2 is notably expressed in the optic vesicle and later in the retina, as well as in the otic vesicle, which later develops into the semicircular canals of the inner ear. Mutations in PAX2 can lead to renal hypoplasia and various ophthalmologic conditions, such as retinal coloboma and microphthalmia, underscoring PAX2′s significance in both renal and ocular health. PAX2 is located on human chromosome 10q24.3-q25.1, and understanding PAX2′s function and interactions is vital for elucidating the mechanisms underlying developmental disorders.

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Pax-2/5/8 Antibody (G-3) References:

1. Paired box genes, PAX-2 and PAX-8, are not frequently mutated in Wilms tumor.  |  Tamimi, Y., et al. 2006. Mutat Res. 601: 46-50. PMID: 16814811
2. Coordinated expression of Pax-5 and FAK1 in metastasis.  |  Crapoulet, N., et al. 2011. Anticancer Agents Med Chem. 11: 643-9. PMID: 21707507
3. Value of PAX 8 immunostaining in tumor diagnosis: a review and update.  |  Ordóñez, NG. 2012. Adv Anat Pathol. 19: 140-51. PMID: 22498579
4. Immunohistochemical distinction of renal cell carcinoma from other carcinomas with clear-cell histomorphology: utility of CD10 and CA-125 in addition to PAX-2, PAX-8, RCCma, and adipophilin.  |  Mentrikoski, MJ., et al. 2014. Appl Immunohistochem Mol Morphol. 22: 635-41. PMID: 25279712
5. Induction of TTF-1 or PAX-8 expression on proliferation and tumorigenicity in thyroid carcinomas.  |  Dupain, C., et al. 2016. Int J Oncol. 49: 1248-58. PMID: 27573549
6. Revisiting PAX-8's Labeling of Merkel Cell Carcinoma.  |  Wang, DP., et al. 2017. Am J Dermatopathol. 39: 873-874. PMID: 28002100
7. C-terminal activating and inhibitory domains determine the transactivation potential of BSAP (Pax-5), Pax-2 and Pax-8.  |  Dörfler, P. and Busslinger, M. 1996. EMBO J. 15: 1971-82. PMID: 8617244
8. Pax-2, kidney development, and oncogenesis.  |  Dressler, GR. 1996. Med Pediatr Oncol. 27: 440-4. PMID: 8827071
9. Conserved biological function between Pax-2 and Pax-5 in midbrain and cerebellum development: evidence from targeted mutations.  |  Schwarz, M., et al. 1997. Proc Natl Acad Sci U S A. 94: 14518-23. PMID: 9405645
10. Sponge Pax cDNA related to Pax-2/5/8 and ancient gene duplications in the Pax family.  |  Hoshiyama, D., et al. 1998. J Mol Evol. 47: 640-8. PMID: 9847404

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pax-2/5/8 Antibody (G-3) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-Pax-2/5/8 Antibody (G-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pax-2/5/8 Antibody (G-3) Specifications

Alternate Product Name: Pax-2/5/8

Product Clone Name: G-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 21-59

Epitope Target: Pax-2

Epitope Species: human

Product Reactivity/Detection: Pax-2, Pax-5 and Pax-8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine, canine, bovine, porcine and avian

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for Pax-2/5/8 Antibody (G-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent

The preferred Pax-5 antibody is Pax-5 Antibody (A-11): sc-13146.