Pax-5 Antibody (A-11): sc-13146

Pax-5 Antibody (A-11) is a mouse monoclonal IgG2a lambda light chain antibody that detects Pax-5 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Pax-5 (A-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase, phycoerythrin, fluorescein isothiocyanate, and multiple Alexa Fluor® conjugates. Pax-5 plays a crucial role in B cell development, acting as a transcription factor that regulates B lymphocyte differentiation at various stages, including pro-B, pre-B, and mature B cells, while being absent in plasma cells. Such regulation maintains balance between immunoglobulin secretion and B cell proliferation, essential for robust immune response. Pax-5 participates in several signaling pathways influencing cell fate decisions during embryogenesis and in adult tissues. Mutations or dysregulation of PAX5 gene have been associated with various hematological malignancies, including small lymphocytic lymphomas, where translocations can lead to aberrant expression of Pax-5, highlighting significance in both normal immune function and disease pathology.

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Pax-5 Antibody (A-11) References:

1. Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis.  |  Adams, B., et al. 1992. Genes Dev. 6: 1589-607. PMID: 1516825
2. Coordinated expression of Pax-5 and FAK1 in metastasis.  |  Crapoulet, N., et al. 2011. Anticancer Agents Med Chem. 11: 643-9. PMID: 21707507
3. The Pax-5 gene: a pluripotent regulator of B-cell differentiation and cancer disease.  |  O'Brien, P., et al. 2011. Cancer Res. 71: 7345-50. PMID: 22127921
4. PAX5-driven subtypes of B-progenitor acute lymphoblastic leukemia.  |  Gu, Z., et al. 2019. Nat Genet. 51: 296-307. PMID: 30643249
5. PAX5 epigenetically orchestrates CD58 transcription and modulates blinatumomab response in acute lymphoblastic leukemia.  |  Li, Y., et al. 2022. Sci Adv. 8: eadd6403. PMID: 36516256
6. The transcription factor PAX5 activates human LINE1 retrotransposons to induce cellular senescence.  |  Tang, H., et al. 2024. EMBO Rep. 25: 3263-3275. PMID: 38866979
7. Chromosomal localization of seven PAX genes and cloning of a novel family member, PAX-9.  |  Stapleton, P., et al. 1993. Nat Genet. 3: 292-8. PMID: 7981748
8. C-terminal activating and inhibitory domains determine the transactivation potential of BSAP (Pax-5), Pax-2 and Pax-8.  |  Dörfler, P. and Busslinger, M. 1996. EMBO J. 15: 1971-82. PMID: 8617244
9. Altered expression of Pax-5 gene in human myeloma cells.  |  Mahmoud, MS., et al. 1996. Blood. 87: 4311-5. PMID: 8639790
10. Deregulation of PAX-5 by translocation of the Emu enhancer of the IgH locus adjacent to two alternative PAX-5 promoters in a diffuse large-cell lymphoma.  |  Busslinger, M., et al. 1996. Proc Natl Acad Sci U S A. 93: 6129-34. PMID: 8650231
11. The role of BSAP (Pax-5) in B-cell development.  |  Busslinger, M. and Urbánek, P. 1995. Curr Opin Genet Dev. 5: 595-601. PMID: 8664547
12. B cell lineage-specific activator protein (BSAP). A player at multiple stages of B cell development.  |  Michaelson, JS., et al. 1996. J Immunol. 156: 2349-51. PMID: 8786288

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Pax-5 Antibody (A-11) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-Pax-5 Antibody (A-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Pax-5 Antibody (A-11) Specifications

Alternate Product Name: Pax-5

Product Clone Name: A-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a lambda light chain

Amino Acids: 371-390

Epitope Target: Pax-5

Epitope Species: human

Product Reactivity/Detection: Pax-5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a lambda light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for Pax-5 Antibody (A-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin, X: TransCruz Reagent