patched/PTCH1 Antibody (188G11): sc-23929
- patched/PTCH1 Antibody (188G11) is a mouse monoclonal IgG1 κ, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 1194-1286 of patched of drosophila origin
- patched/PTCH1 Antibody (188G11) is recommended for detection of patched of d origin by WB, IP and IF
- Anti-patched/PTCH1 Antibody (188G11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for patched/PTCH1 Antibody (188G11) for WB applications. These reagents are now offered in bundles with patched/PTCH1 Antibody (188G11) (see ordering information below).
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SEE ALSO...
Patched Antibody (188G11) is a mouse monoclonal IgG1 kappa light chain antibody that detects patched protein of Drosophila origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-patched antibody (188G11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Patched protein, also known as PTCH1, plays a crucial role in hedgehog signaling pathway, which regulates cell growth, differentiation, and tissue patterning during development. Patched (188G11) antibody recognizes PTCH1 primarily in cell membrane, where PTCH1 functions as a receptor for hedgehog ligands, influencing downstream signaling pathways. Proper localization of PTCH1 at cell surface remains vital for mediating segment polarity and ensuring cells receive correct signals for development. Disruptions in PTCH1 function can lead to developmental abnormalities and have been implicated in various cancers, highlighting PTCH1′s significance in both developmental biology and cancer research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
patched/PTCH1 Antibody (188G11) References:
- The genome sequence of Drosophila melanogaster. | Adams, MD., et al. 2000. Science. 287: 2185-95. PMID: 10731132
- The Drosophila patched gene encodes a putative membrane protein required for segmental patterning. | Hooper, JE. and Scott, MP. 1989. Cell. 59: 751-65. PMID: 2582494
- A protein with several possible membrane-spanning domains encoded by the Drosophila segment polarity gene patched. | Nakano, Y., et al. 1989. Nature. 341: 508-13. PMID: 2797178
- Mutations affecting segment number and polarity in Drosophila. | Nüsslein-Volhard, C. and Wieschaus, E. 1980. Nature. 287: 795-801. PMID: 6776413
- Human homolog of patched, a candidate gene for the basal cell nevus syndrome. | Johnson, RL., et al. 1996. Science. 272: 1668-71. PMID: 8658145
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
patched/PTCH1 Antibody (188G11) | sc-23929 | 200 µg/ml | $322.00 | |||
patched/PTCH1 Antibody (188G11): m-IgG Fc BP-HRP Bundle | sc-526637 | 200 µg Ab; 10 µg BP | $361.00 | |||
patched/PTCH1 Antibody (188G11): m-IgG1 BP-HRP Bundle | sc-532010 | 200 µg Ab; 20 µg BP | $361.00 | |||
patched/PTCH1 Antibody (188G11) AC | sc-23929 AC | 500 µg/ml, 25% agarose | $424.00 | |||
patched/PTCH1 Antibody (188G11) HRP | sc-23929 HRP | 200 µg/ml | $322.00 | |||
patched/PTCH1 Antibody (188G11) FITC | sc-23929 FITC | 200 µg/ml | $336.00 | |||
patched/PTCH1 Antibody (188G11) PE | sc-23929 PE | 200 µg/ml | $349.00 | |||
patched/PTCH1 Antibody (188G11) Alexa Fluor® 488 | sc-23929 AF488 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (188G11) Alexa Fluor® 546 | sc-23929 AF546 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (188G11) Alexa Fluor® 594 | sc-23929 AF594 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (188G11) Alexa Fluor® 647 | sc-23929 AF647 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (188G11) Alexa Fluor® 680 | sc-23929 AF680 | 200 µg/ml | $364.00 | |||
patched/PTCH1 Antibody (188G11) Alexa Fluor® 790 | sc-23929 AF790 | 200 µg/ml | $364.00 |