PARP-4 CRISPR/Cas9 KO Plasmid (h): sc-406615
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PARP-4 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PARP-4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PARP-4 Antibody (B-11): sc-515898
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARP-4 CRISPR/Cas9 KO Plasmid (h) | sc-406615 | 20 µg | $397.00 |
Overview
PARP4 encodes poly(ADP-ribose) polymerase family member 4 (PARP-4), an ADP-ribosyltransferase linked to cellular responses to DNA damage and genotoxic stress. PARP-4 is associated with regulation of chromatin dynamics and maintenance of genome integrity through poly(ADP-ribosyl)ation-dependent signaling that interfaces with DNA repair and checkpoint pathways. Altered PARP4 activity or expression has been reported across multiple cancer-related datasets, supporting its relevance for studying tumor-associated DNA damage tolerance and stress adaptation. PARP-4 is also used as a mechanistic node to investigate cross-talk between ADP-ribosylation, replication stress, and transcriptional regulation in human cells.
PARP-4 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PARP4 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PARP4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PARP4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PARP-4 protein expression.
This CRISPR knockout system enables efficient generation of PARP4-deficient cell models for investigation of PARP-4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PARP4 exon(s) critical for PARP-4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PARP4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PARP-4 CRISPR/Cas9 KO Plasmid (h) and PARP-4 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PARP4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PARP-4 HDR Plasmid (h) and PARP-4 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PARP4 homology arms to support homology-directed repair at defined PARP4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.