Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
PARP-10 CRISPR/Cas9 KO Plasmid (m): sc-437160
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- PARP-10 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the PARP-10 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: PARP-10 Antibody (5H11): sc-53858
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARP-10 CRISPR/Cas9 KO Plasmid (m) | sc-437160 | 20 µg | $397.00 |
Overview
Parp10 encodes PARP-10, a mono-ADP-ribosyltransferase that regulates protein function through ADP-ribosylation, influencing protein stability, subcellular localization, and signaling outputs. PARP-10 has been linked to control of DNA damage responses, replication stress tolerance, and chromatin-associated processes, integrating with ubiquitin-dependent protein regulation and other post-translational modification networks. In mouse systems, PARP-10 activity is commonly investigated in the context of genome maintenance, cell-cycle progression, and stress-adaptive signaling that shapes cellular homeostasis. Dysregulation of ADP-ribosylation pathways is broadly associated with aberrant proliferation and inflammatory signaling, making Parp10 a useful node for mechanistic studies of pathway crosstalk in disease-relevant models.
PARP-10 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Parp10 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Parp10 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Parp10 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish PARP-10 protein expression.
This CRISPR knockout system enables efficient generation of Parp10-deficient cell models for investigation of PARP-10 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Parp10 exon(s) critical for PARP-10 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Parp10 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by PARP-10 CRISPR/Cas9 KO Plasmid (m) and PARP-10 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Parp10 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by PARP-10 HDR Plasmid (m) and PARP-10 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Parp10 homology arms to support homology-directed repair at defined Parp10 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.