PARP-10 Antibody (5H11): sc-53858
- PARP-10 Antibody (5H11) is a rat monoclonal IgG1 PARP-10 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against PARP-10 of human origin
- PARP-10 Antibody (5H11) is recommended for detection of PARP-10 of mouse, rat and human origin by WB, IP and IF
- Anti-PARP-10 Antibody (5H11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for PARP-10 Antibody (5H11). This work is in progress.
PARP-10 Antibody (5H11) is a rat monoclonal IgG1 antibody raised against human PARP-10, with reactivity in mouse, rat, and human samples, making anti-PARP-10 antibody (5H11) suitable for western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF) applications. PARP-10, also called ARTD10 and PARP10, is a member of the poly(ADP-ribose) polymerase family, involved in regulating cell proliferation and cellular transformation. Unlike nuclear counterparts, PARP-10 exists in both nucleus and cytoplasm, playing a distinct role in controlling oncogenic signaling pathways. One key interaction of PARP-10 is with oncogene c-Myc, a transcription factor regulating cell growth and division. PARP-10 inhibits c-Myc-mediated transformation by adding ADP-ribose units to target proteins, modulating their activity. Additionally, PARP-10 interacts with E1A, another protein involved in cell cycle control, further contributing to a role in suppressing oncogenic transformation. These interactions highlight PARP-10′s critical function in maintaining cellular homeostasis and preventing unchecked cell proliferation. PARP-10 (5H11) antibody′s ability to detect PARP-10 in various species and experimental setups makes anti-PARP-10 antibody (5H11) a valuable tool for studying regulatory pathways and mechanisms of cell growth inhibition associated with PARP-10.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
PARP-10 Antibody (5H11) References:
- Functions of poly(ADP-ribose) polymerase in controlling telomere length and chromosomal stability. | d'Adda di Fagagna, F., et al. 1999. Nat Genet. 23: 76-80. PMID: 10471503
- A cellular defense pathway regulating transcription through poly(ADP-ribosyl)ation in response to DNA damage. | Vispe, S., et al. 2000. Proc Natl Acad Sci U S A. 97: 9886-91. PMID: 10944198
- DNA excision repair and DNA damage-induced apoptosis are linked to Poly(ADP-ribosyl)ation but have different requirements for p53. | Beneke, R., et al. 2000. Mol Cell Biol. 20: 6695-703. PMID: 10958667
- The world according to PARP. | Smith, S. 2001. Trends Biochem Sci. 26: 174-9. PMID: 11246023
- Functions of poly(ADP-ribose) polymerase (PARP) in DNA repair, genomic integrity and cell death. | Herceg, Z. and Wang, ZQ. 2001. Mutat Res. 477: 97-110. PMID: 11376691
- Importance of poly(ADP-ribose) glycohydrolase in the control of poly(ADP-ribose) metabolism. | Davidovic, L., et al. 2001. Exp Cell Res. 268: 7-13. PMID: 11461113
- Activation of the apoptotic protease CPP32 by cytotoxic T-cell-derived granzyme B. | Darmon, AJ., et al. 1995. Nature. 377: 446-8. PMID: 7566124
- Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. | Lazebnik, YA., et al. 1994. Nature. 371: 346-7. PMID: 8090205
- Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. | Kaufmann, SH., et al. 1993. Cancer Res. 53: 3976-85. PMID: 8358726
- PARP is important for genomic stability but dispensable in apoptosis. | Wang, ZQ., et al. 1997. Genes Dev. 11: 2347-58. PMID: 9308963
- DNA repair: PARP - another guardian angel? | Jeggo, PA. 1998. Curr Biol. 8: R49-51. PMID: 9427640
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
PARP-10 Antibody (5H11) | sc-53858 | 200 µg/ml | $316.00 | |||
PARP-10 Antibody (5H11) AC | sc-53858 AC | 500 µg/ml, 25% agarose | $416.00 | |||
PARP-10 Antibody (5H11) HRP | sc-53858 HRP | 200 µg/ml | $316.00 | |||
PARP-10 Antibody (5H11) FITC | sc-53858 FITC | 200 µg/ml | $330.00 | |||
PARP-10 Antibody (5H11) PE | sc-53858 PE | 200 µg/ml | $343.00 | |||
PARP-10 Antibody (5H11) Alexa Fluor® 488 | sc-53858 AF488 | 200 µg/ml | $357.00 | |||
PARP-10 Antibody (5H11) Alexa Fluor® 546 | sc-53858 AF546 | 200 µg/ml | $357.00 | |||
PARP-10 Antibody (5H11) Alexa Fluor® 594 | sc-53858 AF594 | 200 µg/ml | $357.00 | |||
PARP-10 Antibody (5H11) Alexa Fluor® 647 | sc-53858 AF647 | 200 µg/ml | $357.00 | |||
PARP-10 Antibody (5H11) Alexa Fluor® 680 | sc-53858 AF680 | 200 µg/ml | $357.00 | |||
PARP-10 Antibody (5H11) Alexa Fluor® 790 | sc-53858 AF790 | 200 µg/ml | $357.00 |