PARG Antibody (H-1): sc-398563

PARG Antibody (H-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects PARG protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-PARG antibody (H-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PARG protein plays a crucial role in cellular response to DNA damage by regulating turnover of poly(ADP-ribose) polymers, which are synthesized by poly(ADP-ribose) polymerase (PARP-1) and other related enzymes. This reversible covalent modification is essential for repair of damaged DNA, as PARG helps modulate activity of chromosomal proteins involved in repair process. Under normal growth conditions, PARG is predominantly localized in cytoplasm and is enzymatically active, undergoing cleavage during apoptosis induced by agents such as etoposide, staurosporine, and Fas. This cleavage generates two C-terminal fragments that retain active site necessary for hydrolyzing poly(ADP-ribose). PARG is expressed as a doublet in standard sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under normal conditions, and PARG gene is located on human chromosome 10q11.23.

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PARG Antibody (H-1) References:

1. Assignment of the poly(ADP-ribose) glycohydrolase gene (PARG) to human chromosome 10q11.23 and mouse chromosome 14B by in situ hybridization.  |  Amé, JC., et al. 1999. Cytogenet Cell Genet. 85: 269-70. PMID: 10449915
2. Poly(ADP-ribosyl)ation reactions in the regulation of nuclear functions.  |  D'Amours, D., et al. 1999. Biochem J. 342 (Pt 2): 249-68. PMID: 10455009
3. Caspase-3-mediated processing of poly(ADP-ribose) glycohydrolase during apoptosis.  |  Affar, EB., et al. 2001. J Biol Chem. 276: 2935-42. PMID: 11053413
4. Selective Loss of PARG Restores PARylation and Counteracts PARP Inhibitor-Mediated Synthetic Lethality.  |  Gogola, E., et al. 2018. Cancer Cell. 33: 1078-1093.e12. PMID: 29894693
5. Poly(ADP-Ribose) Glycohydrolase (PARG) vs. Poly(ADP-Ribose) Polymerase (PARP) - Function in Genome Maintenance and Relevance of Inhibitors for Anti-cancer Therapy.  |  Harrision, D., et al. 2020. Front Mol Biosci. 7: 191. PMID: 33005627
6. PARG inhibition limits HCC progression and potentiates the efficacy of immune checkpoint therapy.  |  Yu, M., et al. 2022. J Hepatol. 77: 140-151. PMID: 35157958
7. The interplay of TARG1 and PARG protects against genomic instability.  |  Groslambert, J., et al. 2023. Cell Rep. 42: 113113. PMID: 37676774
8. PARG-deficient tumor cells have an increased dependence on EXO1/FEN1-mediated DNA repair.  |  Andronikou, C., et al. 2024. EMBO J. 43: 1015-1042. PMID: 38360994
9. PARG is essential for Polθ-mediated DNA end-joining by removing repressive poly-ADP-ribose marks.  |  Vekariya, U., et al. 2024. Nat Commun. 15: 5822. PMID: 38987289
10. Intrinsic PARG inhibitor sensitivity is mimicked by TIMELESS haploinsufficiency and rescued by nucleoside supplementation.  |  Coulson-Gilmer, C., et al. 2024. NAR Cancer. 6: zcae030. PMID: 39015544
11. Isolation and characterization of the cDNA encoding bovine poly(ADP-ribose) glycohydrolase.  |  Lin, W., et al. 1997. J Biol Chem. 272: 11895-901. PMID: 9115250
12. Poly(ADP-ribose) glycohydrolase is present and active in mammalian cells as a 110-kDa protein.  |  Winstall, E., et al. 1999. Exp Cell Res. 246: 395-8. PMID: 9925755

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PARG Antibody (H-1) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-PARG Antibody (H-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PARG Antibody (H-1) Specifications

Alternate Product Name: PARG

Product Clone Name: H-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 677-976

Epitope Target: PARG

Epitope Species: human

Product Reactivity/Detection: PARG

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for PARG Antibody (H-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin