Paralemmin CRISPR/Cas9 KO Plasmid (m): sc-422109
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Paralemmin CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Paralemmin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Paralemmin Antibody (D-5): sc-365869
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Paralemmin CRISPR/Cas9 KO Plasmid (m) | sc-422109 | 20 µg | $397.00 |
Overview
Palm encodes paralemmin, a membrane-associated phosphoprotein enriched in neurons that regulates plasma membrane dynamics, filopodia formation, and neurite outgrowth through lipid-anchored scaffolding at the cell cortex. Paralemmin participates in cytoskeletal remodeling and synaptic maturation processes that shape neuronal connectivity and activity-dependent plasticity. In mouse, Palm expression is linked to developmental patterning of neuronal processes and the organization of dendritic spines, connecting it to pathways governing membrane trafficking, actin remodeling, and synapse structure. Dysregulated paralemmin-associated membrane and synaptic architecture has been studied in the context of neurodevelopmental and neurodegenerative phenotypes where altered connectivity and spine morphology are common experimental readouts.
Paralemmin CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Palm gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Palm together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Palm open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Paralemmin protein expression.
This CRISPR knockout system enables efficient generation of Palm-deficient cell models for investigation of Paralemmin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Palm exon(s) critical for Paralemmin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Palm genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Paralemmin CRISPR/Cas9 KO Plasmid (m) and Paralemmin CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Palm locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Paralemmin HDR Plasmid (m) and Paralemmin HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Palm homology arms to support homology-directed repair at defined Palm target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.