Parafibromin Antibody (E-12): sc-271820
- Parafibromin Antibody (E-12) is a mouse monoclonal IgG3 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 253-297 within an internal region of Parafibromin of human origin
- recommended for detection of Parafibromin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- See Parafibromin (2H1): sc-33638 for Parafibromin antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG3 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Parafibromin Antibody (E-12) for WB applications. These reagents are now offered in bundles with Parafibromin Antibody (E-12) (see ordering information below).
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Parafibromin Antibody (E-12) is a mouse monoclonal IgG3 antibody that detects Parafibromin in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Parafibromin plays a critical role as a tumor suppressor protein, encoded by the CDC73 gene, which is essential for regulating cell division and maintaining genomic stability. Parafibromin is located in the nucleus, where anti-Parafibromin antibody (E-12) identifies a key component of the RNA polymerase II/Paf1 complex, facilitating transcriptional regulation of genes by modifying histones. Proper functioning of Parafibromin is vital, as mutations or dysregulation can lead to hyperparathyroidism and parathyroid carcinoma, making Parafibromin monoclonal antibody (E-12) an important marker for diagnosing these conditions. Additionally, Parafibromin interacts with various proteins involved in transcriptional regulation and chromatin remodeling, underscoring Parafibromin′s significance in cellular processes and potential as a therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Parafibromin Antibody (E-12) References:
- The parafibromin tumor suppressor protein interacts with actin-binding proteins actinin-2 and actinin-3. | Agarwal, SK., et al. 2008. Mol Cancer. 7: 65. PMID: 18687124
- The parafibromin tumor suppressor protein inhibits cell proliferation by repression of the c-myc proto-oncogene. | Lin, L., et al. 2008. Proc Natl Acad Sci U S A. 105: 17420-5. PMID: 18987311
- Absence of nucleolar parafibromin immunoreactivity in subsets of parathyroid malignant tumours. | Juhlin, CC., et al. 2011. Virchows Arch. 459: 47-53. PMID: 21221636
- Parafibromin expression is an independent prognostic factor for colorectal carcinomas. | Zheng, HC., et al. 2011. Hum Pathol. 42: 1089-102. PMID: 21315421
- Parafibromin expression in lung normal tissue and carcinoma: its comparison with clinicopathological parameters of carcinoma. | Xia, P., et al. 2011. Histol Histopathol. 26: 1039-47. PMID: 21692036
- Parafibromin Staining Characteristics in Urothelial Carcinomas and Relationship with Prognostic Parameters. | Karaarslan, S., et al. 2015. J Pathol Transl Med. 49: 389-95. PMID: 26395176
- Dephosphorylated parafibromin is a transcriptional coactivator of the Wnt/Hedgehog/Notch pathways. | Kikuchi, I., et al. 2016. Nat Commun. 7: 12887. PMID: 27650679
- Parafibromin-deficient (HPT-JT Type, CDC73 Mutated) Parathyroid Tumors Demonstrate Distinctive Morphologic Features. | Gill, AJ., et al. 2019. Am J Surg Pathol. 43: 35-46. PMID: 29324469
- Filamin A and parafibromin expression in parathyroid carcinoma. | Storvall, S., et al. 2021. Eur J Endocrinol. 185: 803-812. PMID: 34606470
- Insights into Hyperparathyroidism-Jaw Tumour Syndrome: From Endocrine Acumen to the Spectrum of CDC73 Gene and Parafibromin-Deficient Tumours. | Gheorghe, AM., et al. 2024. Int J Mol Sci. 25: PMID: 38396977
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Parafibromin Antibody (E-12) | sc-271820 | 200 µg/ml | $316.00 | |||
Parafibromin Antibody (E-12): m-IgGκ BP-HRP Bundle | sc-535049 | 200 µg Ab; 40 µg BP | $354.00 | |||
Parafibromin Antibody (E-12): m-IgG3 BP-HRP Bundle | sc-550305 | 200 µg Ab; 40 µg BP | $354.00 | |||
Parafibromin (E-12) Neutralizing Peptide | sc-271820 P | 100 µg/0.5 ml | $68.00 |